Serum metabolome and targeted bile acid profiling reveals potential novel biomarkers for drug-induced liver injury
Serum metabolome and targeted bile acid profiling reveals potential novel biomarkers for drug-induced liver injury
复制标题
DOI：
10.1097/md.0000000000016717
复制
发表时间：
2019-08-01
期刊：
影响因子：
1.6
通讯作者：
Niu, Junqi
中科院分区：
文献类型：
--
作者：
Ma, Zhenhua;Wang, Xiaomei;Niu, Junqi
本研究旨在为药物性肝损伤（DILI）的诊断提供无创、可靠、灵敏的生化参数。采用超高效液相色谱-串联质谱（UHPLC-MS/MS）和选择性反应监测（SRM）分别对38例DILI患者和30例健康对照者的血清代谢组进行分析，并定量15种靶向胆汁酸代谢物。对研究参与者的血清代谢组谱的比较显示DILI患者和健康对照组之间存在显著差异。具体而言，DILI患者血清棕榈酸、牛磺酸去氧胆酸、糖胆酸（GCA）和牛磺酸去氧胆酸（TUDCA）水平显著高于健康对照组，血清溶血磷脂酰乙醇胺水平显著低于健康对照组(P
This study aims to determine the non-invasive, reliable and sensitive biochemical parameters for the diagnosis of drug-induced liver injury (DILI).Ultra-high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) and selected reaction monitoring (SRM) were used to profile the serum metabolome and quantify 15 targeted bile acid metabolites, respectively, in samples obtained from 38 DILI patients and 30 healthy controls.A comparison of the resulting serum metabolome profiles of the study participants revealed significant differences between DILI patients and healthy controls. Specifically, serum palmitic acid, taurochenodeoxycholic acid, glycocholic acid (GCA), and tauroursodeoxycholic acid (TUDCA) levels were significantly higher, and serum lysophosphatidylethanolamine levels were significantly lower in DILI patients vs healthy controls (P