Overexpression of Os2H16 enhances resistance to phytopathogens and tolerance to drought stress in rice
Overexpression of Os2H16 enhances resistance to phytopathogens and tolerance to drought stress in rice
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Os2H16的过度表达增强了水稻对植物病原体的抗性和对干旱胁迫的耐受性
DOI：
10.1007/s11240-013-0374-3
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发表时间：
2013-12-01
影响因子：
3
通讯作者：
Chu, Zhaohui
中科院分区：
文献类型：
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作者：
Li, Ning;Kong, Lingguang;Chu, Zhaohui
水稻Os2H16基因是一个功能未知的基因，在黄单胞菌侵染后表达上调。米。在这项研究中，Os2H16的表达模式进行了分析，证明Os2H16的表达显着诱导细菌和真菌感染以及干旱胁迫，但抑制盐处理。为了进一步研究Os2H16在植物对非生物和生物胁迫的防御反应中的作用，开发了转基因水稻品系。与野生型水稻相比，过量表达Os2H16的转基因水稻表现出对白叶枯病和纹枯病的抗性增强。和立枯丝核菌（Rhizoctonia solani）。相反，Os2 H16敲除系对这两种病原体更敏感。与它们的个体表型一致，接种后，防御相关标记基因的表达在Os2H16过表达个体中比在野生型中升高，而它们在Os2H16敲除系中显著降低。我们还表明，Os2H16过表达株系表现出增强的耐旱性和干旱相关基因的诱导，相比野生型水稻。Os2H16基因敲除株系对干旱胁迫更敏感，干旱相关基因的诱导减少。本研究首次对水稻Os2H16基因的功能进行了鉴定，并表明Os2H16基因正调控植物对非生物胁迫和生物胁迫的防御。
Os2H16, a rice gene of unknown function, has been previously reported to be upregulated in response to infection by Xanthomonas oryzae pv. oryzae. In this study, expression patterns of Os2H16 were analyzed, demonstrating that expression of Os2H16 was dramatically induced by both bacterial and fungal infection as well as by drought stress, but repressed by salt treatment. To further investigate the role of Os2H16 in plant defense responses to abiotic and biotic stresses, transgenic lines of rice were developed. In comparison with wild-type rice, transgenic lines overexpressing Os2H16 show enhanced tolerance to bacterial blight and sheath blight disease, respectively caused by Xanthomonas oryzae pv. oryzae and Rhizoctonia solani. On the contrary, Os2H16 knockdown lines were more susceptible to both pathogens. Consistent with their individual phenotypes, upon inoculation, the expression of defense-related marker genes were elevated in Os2H16 overexpression individuals than in wild-type, while they were significantly reduced in Os2H16 knockdown lines. We also show that Os2H16 overexpression lines display enhanced tolerance to drought stress and elevated induction of drought-related genes, compared to wild-type rice. Os2H16 knockdown lines were more sensitive to drought stress and exhibited reduced induction of drought-related genes. Our study provides the first functional characterization of the rice Os2H16 gene, and suggests that Os2H16 positively modulate plant defense to abiotic and biotic stress.