Mei-P26 regulates the maintenance of ovarian germline stem cells by promoting BMP signaling
Mei-P26 regulates the maintenance of ovarian germline stem cells by promoting BMP signaling
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DOI：
10.1242/dev.077412
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发表时间：
2012-05-01
期刊：
影响因子：
4.6
通讯作者：
Buszczak, Michael
中科院分区：
文献类型：
--
作者：
Li, Yun;Maines, Jean Z.;Buszczak, Michael
在果蝇卵巢中，骨形态发生蛋白（BMP）配体维持生殖干细胞（GSC）处于未分化状态。GSC内BMP通路的激活导致分化因子弹珠袋（bag of marbles，bam）的转录抑制。Nanos-Pumilio翻译抑制复合物和miRNA途径也有助于促进GSC自我更新。如何协调不同的转录和翻译调节剂的活动，以保持GSC在未分化状态仍然不确定。这里提供的数据表明，Mei-P26细胞自主调节GSC维持除了其先前描述的促进生殖系囊肿发育的作用。在未分化的生殖细胞内，Mei-P26与miRNA通路组分相关联并抑制共享靶mRNA的翻译，这表明Mei-P26可以在特定情况下增强miRNA介导的沉默。此外，破坏mei-P26损害BMP信号传导，导致bam在紧邻帽细胞龛的生殖细胞中的不适当表达。Mei-P26的缺失导致BMP拮抗剂Brat在生殖系干细胞中的过早翻译。这些数据表明，Mei-P26在卵巢中具有不同的功能，并参与调节GSC及其分化后代的命运。
In the Drosophila ovary, bone morphogenetic protein (BMP) ligands maintain germline stem cells (GSCs) in an undifferentiated state. The activation of the BMP pathway within GSCs results in the transcriptional repression of the differentiation factor bag of marbles (bam). The Nanos-Pumilio translational repressor complex and the miRNA pathway also help to promote GSC self-renewal. How the activities of different transcriptional and translational regulators are coordinated to keep the GSC in an undifferentiated state remains uncertain. Data presented here show that Mei-P26 cell-autonomously regulates GSC maintenance in addition to its previously described role of promoting germline cyst development. Within undifferentiated germ cells, Mei-P26 associates with miRNA pathway components and represses the translation of a shared target mRNA, suggesting that Mei-P26 can enhance miRNA-mediated silencing in specific contexts. In addition, disruption of mei-P26 compromises BMP signaling, resulting in the inappropriate expression of bam in germ cells immediately adjacent to the cap cell niche. Loss of mei-P26 results in premature translation of the BMP antagonist Brat in germline stem cells. These data suggest that Mei-P26 has distinct functions in the ovary and participates in regulating the fates of both GSCs and their differentiating daughters.