Association of merkel cell polyomavirus infection with morphologic differences in merkel cell carcinoma
Association of merkel cell polyomavirus infection with morphologic differences in merkel cell carcinoma
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DOI：
10.1016/j.humpath.2010.09.011
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发表时间：
2011-05-01
期刊：
影响因子：
3.3
通讯作者：
Hayashi, Kazuhiko
中科院分区：
文献类型：
--
作者：
Kuwamoto, Satoshi;Higaki, Hiromi;Hayashi, Kazuhiko
最近，研究表明，大约80%的默克尔细胞癌含有一种名为默克尔细胞多瘤病毒的新型多瘤病毒，该病毒被认为是一种致癌物。然而，目前还没有完全阐明默克尔细胞癌是否因存在或不存在默克尔细胞多瘤病毒而不同。为了解决这个问题，我们通过形态计量学研究了默克尔细胞多瘤病毒阳性和阴性默克尔细胞癌之间的形态学差异。应用聚合酶链反应和实时定量聚合酶链反应，在26例默克尔细胞癌中检测到默克尔细胞多瘤病毒20例（77%），其中默克尔细胞癌合并鳞状细胞癌4例。有趣的是，梅克尔细胞多瘤病毒仅在普通（纯）梅克尔细胞癌中检测到；4例合并梅克尔细胞癌+鳞状细胞癌均无梅克尔细胞多瘤病毒阳性（P = .001）。形态学分析显示，默克尔细胞多瘤病毒阴性的默克尔细胞癌比默克尔细胞多瘤病毒阳性的默克尔细胞癌具有更不规则的细胞核（P < 0.001）和更丰富的细胞质（P = 0.001），而默克尔细胞多瘤病毒阳性的默克尔细胞癌具有均匀的圆形细胞核和较少的细胞质。形态学测量的可靠性通过观察者内部和观察者之间的信度测试来证实。这些结果表明，默克尔细胞多瘤病毒阳性和阴性的默克尔细胞癌在肿瘤细胞形态上存在统计学上的显著差异，并再次证实合并肿瘤中没有默克尔细胞多瘤病毒。此外，结果强烈提示默克尔细胞多瘤病毒阳性和阴性默克尔细胞癌之间存在根本的生物学差异，支持默克尔细胞多瘤病毒在默克尔细胞多瘤病毒阳性默克尔细胞癌的发病机制中起重要作用。(C) 2011爱思唯尔公司版权所有。
Recently, it has been shown that approximately 80% of Merkel cell carcinomas harbor a novel polyomavirus named Merkel cell polyomavirus, thought to be a carcinogenic agent. However, it is not fully elucidated whether Merkel cell carcinomas differ with regard to the presence or absence of Merkel cell polyomavirus. To address this, we investigated morphologic differences between Merkel cell polyomavirus positive and negative Merkel cell carcinomas by morphometry. Using polymerase chain reaction and real-time quantitative polymerase chain reaction, Merkel cell polyomavirus was detected in 20 (77%) of 26 Merkel cell carcinoma cases, including 4 Merkel cell carcinomas combined with squamous cell carcinomas. Interestingly, Merkel cell polyomavirus was detected only in ordinary (pure) Merkel cell carcinomas; none of the 4 combined Merkel cell carcinomas + squamous cell carcinomas was positive for Merkel cell polyomavirus (P = .001). Morphometric analyses revealed that Merkel cell polyomavirus negative Merkel cell carcinomas had more irregular nuclei (P < .001) and more abundant cytoplasm (P = .001) than Merkel cell polyomavirus positive Merkel cell carcinomas, which had uniform round nuclei and scant cytoplasm. Reliability of the morphometry was confirmed using intraobserver and interobserver reliability tests. These results demonstrated statistically significant differences in tumor cell morphology between Merkel cell polyomavirus positive and negative Merkel cell carcinomas and reconfirmed the absence of Merkel cell polyomavirus in combined tumors. Furthermore, the results strongly suggest fundamental biological differences between Merkel cell polyomavirus positive and negative Merkel cell carcinomas, supporting that Merkel cell polyomavirus plays an important role in the pathogenesis of Merkel cell polyomavirus positive Merkel cell carcinoma. (C) 2011 Elsevier Inc. All rights reserved.