RIP-JIP60 alters conformation of ribosomes in vivo.

RIP-JIP60 alters conformation of ribosomes in vivo.
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RIP-JIP60 改变体内核糖体的构象。

DOI:
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发表时间:
1999
期刊:
Biochemical and Biophysical Research Communications - BBRC
影响因子:
--
通讯作者:
E. Goerschen
E. Goerschen
中科院分区:
--
文献类型:
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作者:
M. Dunaeva;E. Goerschen

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最近的研究表明,在转基因烟草中表达核糖体失活蛋白JIP 60可导致大rRNA的脱嘌呤和植物核糖体的失活。用低浓度的α-八叠球菌素处理转基因烟草植物的核糖体导致rRNA特异性片段的出现。在相同条件下,野生型植物的核糖体没有观察到片段。推测转基因烟草中核糖体构象的改变是由于JIP 60的表达引起的。
Recently it has been demonstrated that expression putative ribosome-inactivating protein JIP 60 in transgenic tobacco plants leads to the depurination of large rRNA and inactivation of plant ribosomes. Treatment of ribosomes from transgenic tobacco plants with low concentration of alpha-sarcin resulted in the appearance of an rRNA specific fragment. No fragment was observed under the same conditions for ribosomes from wild type plants. The alteration of the comformation of ribosomes in transgenic tobacco due to the expression of JIP60 is assumed.
DOI: 10.1016/s0021-9258(18)34241-8
发表时间: 1982-08
期刊: The Journal of biological chemistry
影响因子: --
作者:
Y. Endo;I. Wool
通讯作者: Y. Endo;I. Wool