Safety Assessment of the Candidate Oral Probiotic Lactobacillus Crispatus YIT 12319: Analysis of Antibiotic Resistance and Virulence-Associated Genes
Safety Assessment of the Candidate Oral Probiotic Lactobacillus Crispatus YIT 12319: Analysis of Antibiotic Resistance and Virulence-Associated Genes
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候选口服益生菌卷曲乳杆菌 YIT 12319 的安全性评估：抗生素耐药性和毒力相关基因分析
DOI：
10.1016/j.fct.2020.111278
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Okumura T.
中科院分区：
文献类型：
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作者：
Terai T;Kato K;Ishikawa E;Nakao M;Ito M;Miyazaki K;Kushiro A;Imai S;Nomura Y;Hanada N;Okumura T.
从健康受试者的口腔中分离出卷曲乳杆菌YIT 12319（LcY）作为对口腔健康具有潜在益处的新候选益生菌。作为LcY的安全性评估，我们使用基因组序列草图进行了抗生素敏感性试验和毒力相关基因分析。根据欧洲食品安全局推荐的国际乳制品联合会/国际标准化组织的标准方法，分析了对15种抗生素的敏感性。结果表明，LcY的最低抑菌浓度并不高于其它L.这些菌株对任何抗生素都没有获得抗性，表明LcY没有外部获得的可传播的抗生素抗性基因。使用LcY的基因组草图分析毒力相关基因发现，LcY中潜在的毒力相关基因比其他益生菌少。这些发现表明LcY可能是基于其安全性的候选益生菌。
Lactobacillus crispatusYIT 12319 (LcY) was isolated from the oral cavity of a healthy subject as a new candidate probiotic with potential benefits for oral health. As a safety assessment of LcY, we performed an antibiotic susceptibility test and virulence-associated gene analysis using a draft genome sequence. Susceptibility to 15 antibiotics was analyzed according to the standard method of the International Dairy Federation/International Organization for Standardization, as recommended by the European Food Safety Authority. The results showed that the minimum inhibitory concentrations of LcY were not higher than those of otherL. crispatusstrains, which have not acquired resistance to any antibiotics, suggesting that LcY had no externally acquired transmissible antibiotic resistance genes. Analysis of virulence-associated genes using the draft genome of LcY found that there were fewer potential virulence-associated genes in LcY than in other probiotics. These findings suggest that LcY could be a candidate probiotic based on its safety profile.