Carbonic anhydrase II mRNA is induced in rabbit kidney cortex during chronic metabolic acidosis.
Carbonic anhydrase II mRNA is induced in rabbit kidney cortex during chronic metabolic acidosis.
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慢性代谢性酸中毒期间，兔肾皮质中会诱导碳酸酐酶 II mRNA。
DOI：
10.1152/ajprenal.1993.265.6.f764
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
Bargiello,T
中科院分区：
文献类型：
--
作者：
Schwartz,GJ;Winkler,CA;Zavilowitz,BJ;Bargiello,T
碳酸酐酶II （CA II）是肾中主要的碳酸酐酶异构体，被认为主要存在于近端小管和集管嵌入细胞的细胞质中。碳酸酐酶通过在CO2存在下催化OH-生成HCO3-来促进H+的分泌。我们已经证明，慢性代谢性酸中毒4-6天期间，肾皮质CA II活性受到刺激Brion， B.J. Zavilowitz， O. Rosen和G.J. Schwartz。点。[j].中国生物医学工程学报，2016,32(2):387 - 398。这些研究的目的是研究在类似条件下CA II mRNA的调控。通过逆转录兔肾总RNA获得兔CA II cDNA的主要部分，然后利用从人、小鼠和鸡CA II cDNA编码区的保守区域制备的寡核苷酸引物进行聚合酶链反应（RT-PCR）扩增。对696 bp的RT-PCR产物进行测序，发现71-86%与其他三个物种的CA II cdna同源。推导出的氨基酸序列与先前对兔红细胞CA II的Edman分析结果非常吻合（> 97%）。Northern分析显示表达约1.4 kb RNA，皮层>外髓质>内髓质。当归一化到β -肌动蛋白或苹果酸脱氢酶的表达时，酸处理兔肾皮质的稳态mRNA表达量约为对照组的两倍。酸处理3 d后CAⅱmRNA的刺激大于酸处理5 ~ 6 d后。（摘要删节250字）
Carbonic anhydrase II (CA II), the predominant isoform of carbonic anhydrase in the kidney, is believed to be localized primarily in the cytoplasm of proximal tubule and collecting duct intercalated cells. Carbonic anhydrase facilitates H+ secretion by catalyzing the formation of HCO3- from OH- in the presence of CO2. We have shown that renal cortical CA II activity is stimulated during 4-6 days of chronic metabolic acidosis [L.P. Brion, B.J. Zavilowitz, O. Rosen, and G.J. Schwartz. Am. J. Physiol. 261 (Regulatory Integrative Comp. Physiol. 30): R1204-R1213, 1991]. The purpose of these studies was to examine under similar conditions the regulation of CA II mRNA. We obtained a major portion of the rabbit CA II cDNA by reverse transcription of total RNA from rabbit kidney followed by amplification using oligonucleotide primers prepared from conserved areas in the coding regions of human, mouse, and chick CA II cDNAs in a polymerase chain reaction (RT-PCR). The 696-bp RT-PCR product was sequenced and found to be 71-86% homologous to CA II cDNAs from the other three species. The deduced amino acid sequence agreed closely (> 97%) with a previous Edman analysis of rabbit erythrocyte CA II. Northern analysis showed expression of a approximately 1.4 kb RNA, with cortex > outer medulla > inner medulla. Steady-state mRNA expression from kidney cortex of acid-treated rabbits was about twice that from controls, when normalized to the expression of beta-actin or malate dehydrogenase. The stimulation of CA II mRNA was greater after 3 days than after 5-6 days of acid treatment. (ABSTRACT TRUNCATED AT 250 WORDS)