Human serum albumin and oxidative stress in preeclamptic women and the mechanism of albumin for stress reduction.
Human serum albumin and oxidative stress in preeclamptic women and the mechanism of albumin for stress reduction.
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DOI：
10.1016/j.heliyon.2017.e00369
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发表时间：
2017-08
期刊：
影响因子：
4
通讯作者：
Wakatsuki A
中科院分区：
文献类型：
--
作者：
Kinoshita H;Watanabe K;Azma T;Feng GG;Akahori T;Hayashi H;Sato M;Fujiwara Y;Wakatsuki A
本研究旨在解决先兆子痫的机制之一，研究氧化应激水平、人血清白蛋白和内皮功能是否与孕妇相关，以及人血清白蛋白是否降低人血管平滑肌细胞中NADPH氧化酶激活产生的超氧化物水平。选取子痫前期组（n = 33）和非子痫前期组（n = 37）孕妇，测定其血清活性氧水平（d-ROM）和血流介导的血管舒张功能（FMD）。对人冠状动脉平滑肌细胞或网膜动脉进行等长力记录、超氧化物水平、Western免疫印迹和免疫组织化学评价。超氧化物清除试验也在无细胞系统中进行。子痫前期组FMD较正常组低，血清d-ROM值较正常组高。血清d-ROM水平与FMD程度呈负相关，血清白蛋白水平与d-ROM水平呈负相关。D-葡萄糖可降低左克罗卡林诱导的人网膜动脉扩张，并增加超氧化物水平和人冠状动脉平滑肌细胞中NADPH氧化酶亚基p47 phox的募集。人血清白蛋白（0.05至0.5克/分升）防止这些变化，而它没有产生超氧化物清除作用。孕妇血清白蛋白与氧化应激呈负相关，与内皮功能呈正相关。人血清白蛋白似乎通过抑制人血管平滑肌中的NADPH氧化酶来降低氧化应激，表明血清水平可能是某些人类疾病中血管氧化应激的关键决定因素。
The present study to address one of the mechanisms in preeclampsia, examined whether levels of oxidative stress, human serum albumin, and endothelial function correlate in pregnant women and whether human serum albumin reduces levels of superoxide produced by NADPH oxidase activation in the human vascular smooth muscle cells. Pregnant women with (Preeclampsia group, n = 33) and without preeclampsia (Normal group, n = 37) were recruited to determine levels of reactive oxygen species (serum diacron-reactive oxygen metabolite [d-ROM]), and the flow-mediated dilation (FMD). Human coronary arterial smooth muscle cells or omental arteries were subjected to evaluate isometric force recordings, levels of superoxide, western immunoblotting, and immunohistochemistry. The superoxide scavenging assay was also performed in a cell-free system. Women in the preeclampsia group demonstrated lower FMD and higher serum d-ROM values than those in the normal group. There were the inverse correlations between serum levels of d-ROM and the degree of FMD and between serum levels of albumin and those of d-ROM. D-glucose reduced the levcromakalim-induced dilation of human omental arteries, and it increased levels of superoxide and the recruitment of the NADPH oxidase subunit p47phox in human coronary arterial smooth muscle cells. Human serum albumin (0.05 to 0.5 g/dL) prevented these alterations whereas it exerted no superoxide scavenging effect. Serum albumin relates to oxidative stress inversely, but to the endothelial function positively, in pregnant women. Human serum albumin appears to reduce oxidative stress via NADPH oxidase inhibition in the human vascular smooth muscle, indicating that the serum level may be a critical determinant of vascular oxidative stress in some human diseases.