Quantification of Bovine Milk Oligosaccharides Using Liquid Chromatography-Selected Reaction Monitoring-Mass Spectrometry
Quantification of Bovine Milk Oligosaccharides Using Liquid Chromatography-Selected Reaction Monitoring-Mass Spectrometry
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DOI：
10.1021/jf202035m
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发表时间：
2011-09-28
影响因子：
6.1
通讯作者：
McJarrow, Paul
中科院分区：
文献类型：
--
作者：
Fong, Bertram;Ma, Kevin;McJarrow, Paul
低聚果糖是牛奶的重要成分，具有益生元、抗感染剂和免疫系统调节剂的生物功效，并且是神经功能唾液酸的可能来源。牛乳低聚糖浓度较低，缺乏人乳低聚糖的多样性，但可能是儿科食品中乳低聚糖的商业来源。对于这种开发，需要能够定量寡糖。本研究验证了亲水作用色谱-高效液相色谱-高分辨率选择反应监测-质谱（HILIC HPLC-HRSRM-MS）方法，用于测定牛乳、牛初乳和婴儿配方奶粉中的六种不同寡糖。提取导致高回收率（90-103%），对于两种主要寡糖，3 '-唾液酸乳糖和6'-唾液酸乳糖，重复性变异系数范围为2 - 9%，对于浓度低得多的寡糖，6 '-唾液酸乳糖胺，二唾液酸乳糖和N-乙酰半乳糖胺乳糖，重复性变异系数范围为1 - 17%。在任何样品中均未检测到第六种寡糖3 '-唾液酸乳糖胺。
Oligosaccharides are important components of milk with bioefficacy as prebiotics, anti-infectives, and immune system modulators and as a possible source of sialic acid for neural function. Bovine milk oligosaccharides are lower in concentration and lack the diversity of human milk oligosaccharides but could be a commercial source of milk oligosaccharides for pediatric foods. For this development, an ability to quantify the oligosaccharides is required. This study validated a hydrophilic interaction chromatography high-performance liquid chromatography-high-resolution selected reaction monitoring-mass spectrometry (HILIC HPLC-HRSRM-MS) method for measuring six different oligosaccharides in bovine milk, bovine colostrum, and infant formulas. The extraction resulted in a high recovery (90-103%) with a repeatability coefficient of variation ranging from 2 to 9% for the two dominant oligosaccharides, 3'-sialyllactose and 6'-sialyllactose, and ranging from 1 to 17% for the much lower concentration oligosaccharides, 6'-sialyllactosamine, disialyllactose, and N-acetylgalactosaminyllactose. The sixth oligosaccharide, 3'-sialyllactosamine, was not detected in any of the samples.