Rabbit retinal neurons and glia express a variety of ENaC/DEG subunits
Rabbit retinal neurons and glia express a variety of ENaC/DEG subunits
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DOI：
10.1152/ajpcell.00457.2001
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发表时间：
2002-07-01
影响因子：
5.5
通讯作者：
Keyser, KT
中科院分区：
文献类型：
--
作者：
Brockway, LM;Zhou, ZH;Keyser, KT
在哺乳动物大脑中发现了上皮Na+通道/变性素（ENaC/DEG）离子通道家族的一些成员。因此，我们检测了这些通道在中枢神经系统的另一部分——兔子视网膜中的RNA和蛋白表达。接下来，我们试图证明Muller神经胶质中存在amiloride敏感电流的生理证据，在先前研究的基础上，Muller神经胶质被认为表达α - enac （Golestaneh N, de Kozak Y, Klein C, and Mirshahi M. glia 33: 160-168, 2001）。视网膜RNA的RT-PCR显示存在α -、β -、γ -和δ - enac以及酸感离子通道（ASIC） 1、ASIC2、ASIC3和ASIC4。针对α - enac和β - enac抗体的免疫组织化学定位分别在Muller细胞和神经元中显示标记。Western blotting检测α - enac、β - enac和ASIC1的存在。培养的Muller细胞全细胞膜片夹紧。这些细胞表现出向内的Na+电流，被阿米洛利阻断。这些数据首次证明了兔视网膜中多种ENaC和ASIC亚基的表达，以及神经元和胶质细胞中特定亚基的不同细胞表达模式。
Some members of the epithelial Na+ channel/degenerin (ENaC/DEG) family of ion channels have been detected in mammalian brain. Therefore, we examined the RNA and protein expression of these channels in another part of the central nervous system, the rabbit retina. We next sought to demonstrate physiological evidence for an amiloride-sensitive current in Muller glia, which, on the basis of a previous study, are thought to express alpha-ENaC (Golestaneh N, de Kozak Y, Klein C, and Mirshahi M. Glia 33: 160-168, 2001). RT-PCR of retinal RNA revealed the presence of alpha-, beta-, gamma-, and delta-ENaC as well as acid-sensing ion channel (ASIC) 1, ASIC2, ASIC3, and ASIC4. Immunohistochemical localization with antibodies against alpha-ENaC and beta-ENaC showed labeling in Muller cells and neurons, respectively. The presence of alpha-ENaC, beta-ENaC, and ASIC1 was detected by Western blotting. Cultured Muller cells were whole cell patch clamped. These cells exhibited an inward Na+ current that was blocked by amiloride. These data demonstrate for the first time both the expression of a variety of ENaC and ASIC subunits in the rabbit retina as well as distinct cellular expression patterns of specific subunits in neurons and glia.