Improved High Collection Efficiency in Fluorescence Microscopy with a Weierstrass-Sphere Solid Immersion Lens
Improved High Collection Efficiency in Fluorescence Microscopy with a Weierstrass-Sphere Solid Immersion Lens
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DOI：
10.1143/jjap.41.l858
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发表时间：
2002-07
影响因子：
1.5
通讯作者：
M. Yoshita;Kazuko Koyama;Y. Hayamizu;M. Baba;H. Akiyama
中科院分区：
文献类型：
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作者：
M. Yoshita;Kazuko Koyama;Y. Hayamizu;M. Baba;H. Akiyama
本文研究了用维尔斯特拉斯球固体浸没透镜（SIL）提高荧光显微镜的收集效率。使用折射率n=1.845的维尔斯特拉斯球SIL和数值孔径为0.55的物镜透镜，我们证明了直径为0.11 μ m的染料掺杂微球珠样品的荧光的高收集效率，这是传统显微镜测量的21倍，估计为76%。对维尔斯特拉斯球型锡尔斯的照明均匀性和像差进行了评价。
We investigate improvements of the collection efficiency in fluorescence microscopy with a Weierstrass-sphere solid immersion lens (SIL). Using a Weierstrass-sphere SIL with refractive index n=1.845 and an objective lens with 0.55 numerical aperture, we demonstrate the high collection efficiency of fluorescence from 0.11-µm-diameter dye-doped microsphere bead samples, which is 21 times higher than that measured in conventional microscopy and is estimated to be 76%. Illumination uniformity and aberrations in Weierstrass-sphere SILs are also evaluated.