DIABETES IMPAIRS THE LATE INFLAMMATORY RESPONSE TO WOUND-HEALING
DIABETES IMPAIRS THE LATE INFLAMMATORY RESPONSE TO WOUND-HEALING
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DOI：
10.1016/0022-4804(91)90196-s
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发表时间：
1991-04-01
影响因子：
2.2
通讯作者：
SHIRES, GT
中科院分区：
文献类型：
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作者：
FAHEY, TJ;SADATY, A;SHIRES, GT
糖尿病被认为是伤口愈合受损的危险因素。本研究观察了正常和链脲霉素诱导的糖尿病小鼠创伤腔内白细胞的浸润及肿瘤坏死因子-α (TNF)和IL-6的表达。沿正常小鼠和糖尿病小鼠的侧翼植入含有聚乙烯醇海绵条的穿孔硅树脂伤口腔。分析植入后1、3和7天从腔室抽吸的伤口液中白细胞总数、TNF和IL-6水平。虽然植入后第1天和第3天创液中白细胞数量相似，但在植入后第7天，糖尿病动物创液中的炎症细胞（13.8 × 106/ml）明显少于正常动物创液（28.5 × 106/ml）。两组无细胞渗出液中TNF水平在检查的所有天内相似。两组IL-6水平在植入后第1天和第3天相似，但在植入后第7天，正常动物创面液中IL-6含量（10,998 U/ml）明显高于糖尿病动物创面液（2096 U/ml）。植入后8天的腔室组织学评估显示新生血管减少，肉芽组织组织减少。这些数据表明，在伤口愈合的炎症晚期，糖尿病的延迟愈合与白细胞浸润和伤口液IL-6水平的改变有关。
Diabetes mellitus is recognized as a risk factor for compromised wound healing. This study examines leukocyte infiltration and the appearance of tumor necrosis factor-α (TNF) and IL-6 in wound chambers implanted in normal and streptozotocin-induced diabetic mice. Perforated silicone wound chambers containing a strip of polyvinyl alcohol sponge were implanted along the flanks of normal and diabetic mice. Wound fluid aspirated from the chambers 1, 3, and 7 days following implantation was analyzed for the total number of leukocytes and TNF and IL-6 levels. While the number of leukocytes in the wound fluid was similar on Days 1 and 3 following implantation, there were significantly fewer inflammatory cells in wound fluid from diabetic animals (13.8 × 106/ml) than in wound fluid from normal animals (28.5 × 106/ml) on Day 7 following implantation. TNF levels in the cell-free exudate fluid were similar between the two groups on all days examined. IL-6 levels were similar on Days 1 and 3 following implantation between the two groups, but there was significantly more IL-6 in wound fluid from normal animals (10,998 U/ml) than in wound fluid from diabetic animals (2096 U/ml) on Day 7 following implantation. Histologic evaluation of chambers 8 days following implantation revealed decreased neovascularization and less organization of granulation tissue. These data suggest that delayed healing in diabetes is associated with altered leukocyte infiltration and wound fluid IL-6 levels during the late inflammatory phase of wound healing.