The Engineering of Bacteria Bearing Azido-Pseudaminic Acid-Modified Flagella
The Engineering of Bacteria Bearing Azido-Pseudaminic Acid-Modified Flagella
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DOI：
10.1002/cbic.200900018
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发表时间：
2009-05-25
期刊：
影响因子：
3.2
通讯作者：
Tanner, Martin E.
中科院分区：
文献类型：
--
作者：
Liu, Feng;Aubry, Annie J.;Tanner, Martin E.
抓住老虎的尾巴：我们已经证明了这一点，我们喂食不动的变种C。利用中性叠氮化物标记的伪氨基酸前体，我们可以恢复它们产生功能鞭毛的能力。鞭毛表面的叠氮伪氨基酸的存在提供了一个生物正交的化学柄，可以用来修饰鞭毛蛋白。
Catch a tiger by the tail: We have demonstrated that by feeding nonmotile mutantC. jejunibacteria with a neutral azide‐labelled pseudaminic acid precursor we can restore their ability to generate functional flagella. The presence of azido‐pseudaminic acid on the surface of the flagella provides a bio‐orthogonal chemical handle that can be used to modify the flagellar proteins.