Identification of S23 causing both interspecific hybrid male sterility and environment-conditioned male sterility in rice
Identification of S23 causing both interspecific hybrid male sterility and environment-conditioned male sterility in rice
复制标题
引起水稻种间杂种雄性不育和环境条件雄性不育的S23的鉴定
DOI：
10.1186/s12284-019-0271-4
复制
发表时间：
2019-02-28
期刊：
影响因子：
5.5
通讯作者：
Liu, Ziqiang
中科院分区：
文献类型：
--
作者：
Fang, Chaowei;Li, Le;Liu, Ziqiang
背景：水稻是一种重要的遗传多样性资源，可用于提高水稻产量。然而，杂交不育性严重限制了稻属间的基因交流，阻碍了杂交水稻远缘杂种优势的利用。为了进一步保护这些基因资源，以籼稻品种HJX 74为轮回亲本，以O. glumaepatula作为供体亲本。在SSSL的发育过程中，S_（23）是O. sativa和O. glumaepatula的基因组序列，并将其精细定位于O. sativa和O. glumaepatula，分别编码三个和两个候选ORF。qRT-PCR和序列分析排除了一个共同的ORF作为候选基因。此外，S23的杂种雄性不育性对环境敏感，仅在自然短日（NSD）下才能观察到。本研究对S23的鉴定和候选基因分析为研究水稻种间杂种F1雄性不育与环境条件雄性不育的互作提供了一个有价值的实例，有利于保存和利用野生稻的有利基因或等位基因，并且允许在杂交水稻育种中更有效地利用远缘杂种优势。
Background: Oryza glumaepatula represents an important resource of genetic diversity that can be used to improve rice production. However, hybrid sterility severely restricts gene flow between Oryza species, and hinders the utilization of distant heterosis in hybrid rice breeding.Results: In order to fully exploit the beneficial genes of O. glumaepatula and facilitate the conservation of these gene resources, a set of chromosome single-segment substitution lines (SSSLs) was developed using an indica variety HJX74 as the recurrent parent and an accession of O. glumaepatula as the donor parent. During the process of SSSLs development, S23, a locus conferring hybrid male sterility between O. sativa and O. glumaepatula, was identified and fine mapped to 11.54 kb and 7.08 kb genomic region in O. sativa and O. glumaepatula, respectively, encoding three and two candidate ORFs, respectively. qRT-PCR and sequence analysis excluded one common ORF as the candidate gene. In addition, hybrid male sterility caused by S23 was environment-sensitive, and could be observed only in natural short-day (NSD).Conclusion: Identification and candidate genes analysis of S23 in this study provides a valuable example to study the crosstalk between interspecific F-1 hybrid male sterility and environment-conditioned male sterility in rice, facilitates reserving and utilizing favorable genes or alleles of wild Oryza species, and allows for a more efficient exploitation of distant heterosis in hybrid rice breeding.