Prospective identification, isolation, and systemic transplantation of multipotent mesenchymal stem cells in murine bone marrow.
Prospective identification, isolation, and systemic transplantation of multipotent mesenchymal stem cells in murine bone marrow.
复制标题
小鼠骨髓中多能间充质干细胞的前瞻性鉴定、分离和系统移植。
DOI：
10.1084/jem.20091046
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发表时间：
2009-10-26
期刊：
影响因子：
--
通讯作者：
Matsuzaki Y
中科院分区：
文献类型：
--
作者：
Morikawa S;Mabuchi Y;Kubota Y;Nagai Y;Niibe K;Hiratsu E;Suzuki S;Miyauchi-Hara C;Nagoshi N;Sunabori T;Shimmura S;Miyawaki A;Nakagawa T;Suda T;Okano H;Matsuzaki Y
间充质干细胞（MSCs）被定义为能够在体外持续生长并产生多种间充质细胞系的细胞。由于间充质干细胞仅从培养组织中分离出来，在体内尚未鉴定出相应的细胞，对其生理作用甚至确切的组织位置知之甚少。在这项研究中，我们使用表型、形态学和功能标准从成年小鼠骨髓中鉴定并前瞻性分离出MSCs子集（PDGFRα+Sca-1+CD45−TER119−）。个体间充质干细胞在体内移植后可产生高频率的集落，并可分化为造血生态位细胞、成骨细胞和脂肪细胞。初始间充质干细胞以静止状态驻留在血管周围区域。这项研究提供了一种有效的方法，可以将MSCs定义为体内实体。
Mesenchymal stem cells (MSCs) are defined as cells that undergo sustained in vitro growth and can give rise to multiple mesenchymal lineages. Because MSCs have only been isolated from tissue in culture, the equivalent cells have not been identified in vivo and little is known about their physiological roles or even their exact tissue location. In this study, we used phenotypic, morphological, and functional criteria to identify and prospectively isolate a subset of MSCs (PDGFRα+Sca-1+CD45−TER119−) from adult mouse bone marrow. Individual MSCs generated colonies at a high frequency and could differentiate into hematopoietic niche cells, osteoblasts, and adipocytes after in vivo transplantation. Naive MSCs resided in the perivascular region in a quiescent state. This study provides the useful method needed to identify MSCs as defined in vivo entities.
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影响因子：
15.9
作者：
Hoffmann, A;Pelled, G;Gazit, D
通讯作者：
Gazit, D
影响因子：
20.3
作者：
Beltrami, Antonio R.;Cesselli, Daniela;Beltrami, Carlo A.
通讯作者：
Beltrami, Carlo A.
DOI：
10.1073/pnas.0408239102
发表时间：
2005-03-29
影响因子：
11.1
作者：
Kondo, T;Johnson, SA;Hashino, E
通讯作者：
Hashino, E
影响因子：
5.2
作者：
Koide, Yoko;Morikawa, Satoru;Matsuzaki, Yumi
通讯作者：
Matsuzaki, Yumi
影响因子：
37.8
作者：
Barbash, IM;Chouraqui, P;Leor, J
通讯作者：
Leor, J