Inhibition of Staphylococcus aureus by antimicrobial biofilms formed by competitive exclusion microorganisms on stainless steel.
Inhibition of Staphylococcus aureus by antimicrobial biofilms formed by competitive exclusion microorganisms on stainless steel.
复制标题
通过竞争排斥微生物在不锈钢上形成的抗菌生物膜抑制金黄色葡萄球菌。
DOI：
10.1016/j.ijfoodmicro.2016.09.007
复制
发表时间：
2016
影响因子：
5.4
通讯作者：
J. Ryu
中科院分区：
文献类型：
--
作者：
Hyeri Son;Sunhyun Park;L. Beuchat;Hoikyung Kim;J. Ryu
本研究的目的是开发一种抗干燥的抗菌表面使用的生物膜竞争排斥（CE）微生物抑制金黄色葡萄球菌。我们从土壤、食物和食物接触表面分离出161种对S有抑制作用的微生物。金黄色。其中，3个CE微生物（Streptomycespororaveusstrain Gaeunsan-18，Bacillussafensstrain Chamnamu-sup 5-25，Pseudomonasazotoformansstrain Letterum-9）表现出较强的抗菌活性和较高的生长速度进行评价。这些分离物在浸入25 °C的Bennet肉汤和胰蛋白酶大豆肉汤中的不锈钢试样（SSC）上在24小时内形成生物膜。这些生物膜中的细胞与附着于SSC但未附着在生物膜中的细胞相比，显示出对干燥（43%相对湿度[RH]）的显著（P≤ 0.05）增强的抗性。结果表明，分离菌株在SSCs上形成的生物膜对S.在25 °C和43%RH下测定金黄色。与缺乏CE微生物形成的生物膜的SSC相比，S.金黄色葡萄球菌SSC携带CE生物膜的数量显著减少（P≤ 0.05）。结果表明，持久的抗菌活性对S。aureuson不锈钢表面可以通过存在CE微生物的生物膜来实现。这些信息将有助于制定战略，以提高食品在储存，加工和分销过程中的微生物安全性，促进有效的抗菌食品接触表面的发展。
The goal of this study was to develop a desiccation resistant antimicrobial surface using biofilm of competitive exclusion (CE) microorganism inhibitory to Staphylococcus aureus. We isolated 161 microorganisms from soils, foods, and food-contact surfaces that are inhibitory toS. aureus. Among them, three CE microorganisms (Streptomycesspororaveusstrain Gaeunsan-18,Bacillus safensisstrain Chamnamu-sup 5–25, andPseudomonas azotoformansstrain Lettuce-9) exhibiting strong antibacterial activity and high growth rates were selected for evaluation. These isolates formed biofilms within 24 h on stainless steel coupons (SSCs) immersed in Bennet's broth and tryptic soy broth at 25 °C. Cells in these biofilms showed significantly (P≤ 0.05) enhanced resistance to a desiccation (43% relative humidity [RH]) compared to those attached to SSCs but not in biofilms. The antimicrobial activities of biofilms formed by these isolates on SSCs againstS. aureusat 25 °C and 43% RH were determined. Compared to SSCs lacking biofilms formed by CE microorganisms, populations ofS. aureuson SSCs harboring CE biofilms were significantly lower (P≤ 0.05). Results indicate that persistent antimicrobial activity againstS. aureuson stainless steel surfaces can be achieved by the presence of biofilms of CE microorganisms. This information will be useful when developing strategies to improve the microbiological safety of foods during storage, processing, and distribution by facilitating the development of effective antimicrobial food-contact surfaces.