Detection of lipid radicals using EPR.
Detection of lipid radicals using EPR.
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DOI：
10.1089/152308604773934396
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发表时间：
2004-07
影响因子：
6.6
通讯作者：
S. Venkataraman;Freya Q. Schafer;G. Buettner
中科院分区：
文献类型：
--
作者：
S. Venkataraman;Freya Q. Schafer;G. Buettner
细胞氧化分子以产生能量并制造材料来构建和支持生命所需的结构和功能。然而，不必要的氧化可能会损坏这些相同的结构并损害功能。脂质（膜和脂蛋白中的脂质）是不需要的氧化的目标。这些氧化的主要机制是自由基介导的链式反应。在这里，我们概述了如何使用电子顺磁共振 (EPR) 来检测脂质过氧化过程中形成的自由基。尽管已经实现了脂质衍生自由基的直接检测，但该方法对于检测细胞中的这些自由基并不可行。使用 α-(4-吡啶基-1-氧化物)-N-叔丁基硝酮和 5,5-二甲基-吡咯啉-1-氧化物进行自旋捕获提供了有关细胞脂质过氧化的最多信息。我们提出了通过 EPR 成功检测脂质自由基的一些注意事项。
Cells oxidize molecules to generate energy and to make the materials to build and support the structures and functions needed for life. However, unwanted oxidations can damage these same structures and impair function. Lipids (the lipids in membranes and lipoproteins) are targets of unwanted oxidations. The primary mechanism of these oxidations is free radical-mediated chain reactions. Here we provide an overview of how electron paramagnetic resonance (EPR) can be used to detect the free radicals formed during lipid peroxidation. Although direct detection of lipid-derived radicals has been accomplished, the approach is not feasible for detecting these radicals in cells. Spin trapping with alpha-(4-pyridyl-1-oxide)-N-tert-butylnitrone and 5,5-dimethyl-pyrroline-1-oxide has provided the most information on cellular lipid peroxidation. We present some considerations for successful detection of lipid radicals by EPR.