HEMOLYTIC PROPERTIES OF PLURONIC SURFACTANTS AND EFFECTS OF PURIFICATION
HEMOLYTIC PROPERTIES OF PLURONIC SURFACTANTS AND EFFECTS OF PURIFICATION
复制标题
DOI：
10.3109/10731199509117673
复制
发表时间：
1995-01-01
影响因子：
--
通讯作者：
THOMAS, S
中科院分区：
文献类型：
--
作者：
LOWE, KC;FURMIDGE, BA;THOMAS, S
研究了将小鼠、大鼠、兔或仓鼠的血液与市售级或硅胶纯化的普朗尼克F-68或普朗尼克F-38组分一起孵育的效果。将血液与高达4.0%（w/v）的市售或纯化的Pluronic F-68孵育未产生可检测的溶血（< 0.1%）。当市售Pluronic F-68浓度高于4.0%（w/v）时，确实发生溶血。用10.0%（w/v）Pluronic F-68孵育的大鼠血液中，这是最大的，其中平均溶血为4.7 +/- 1.5%;在用纯化的Pluronic F-68级分孵育后，大鼠血液中的平均溶血降低至0.5 +/- 0.3%（P < 0.05）。当与大鼠或仓鼠血液一起孵育时，商业级或纯化的Plutonic F-38均未产生任何显著的溶血。用10.0%（w/v）市售Pluronic F-38孵育家兔血液仅产生0.5%溶血。
The effects of incubating blood from mice, rats, rabbits or hamsters with either a commercial grade or a silica-purified fraction of Pluronic F-68 or Pluronic F-38 have been studied. Incubation of blood with up to 4.0% (w/v) of commercial or purified Pluronic F-68 produced no detectable haemolysis (< 0.1%). Haemolysis did occur with concentrations of commercial Pluronic F-68 above 4.0% (w/v). This was maximal with rat blood incubated with 10.0% (w/v) Pluronic F-68, where the mean haemolysis was 4.7 +/- 1.5%; the mean haemolysis in rat blood was reduced to 0.5 +/- 0.3% (P < 0.05) following incubation with the purified Pluronic F-68 fraction. Neither commercial grade or purified Plutonic F-38 produced any significant haemolysis when incubated with rat or hamster blood. Incubation of rabbit blood with 10.O% (w/v) commercial Pluronic F-38 produced only 0.5% haemolysis.