Structural and genomic DNA analysis of a putative transcription factor SCO5550 from Streptomyces coelicolor A3 (2) : Regulating the expression of gene sco5551 as a transcriptional activator with a novel diner shape
Structural and genomic DNA analysis of a putative transcription factor SCO5550 from Streptomyces coelicolor A3 (2) : Regulating the expression of gene sco5551 as a transcriptional activator with a novel diner shape
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天蓝色链霉菌 A3 的假定转录因子 SCO5550 的结构和基因组 DNA 分析 (2)：调节 sco5551 基因的表达，作为具有新型晚餐形状的转录激活剂
DOI：
10.1016/j.bbrc.2013.04.017
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发表时间：
2013
影响因子：
3.1
通讯作者：
Min Yao
中科院分区：
文献类型：
--
作者：
Takeshi Hayashi;Yoshikazu Tanaka;Naoki Sakai;Nobuhisa Watanabe;Tomohiro Tamura;Isao Tanaka;Min Yao
SCO 5550来自于模式放线菌天蓝色链霉菌A3（2），被鉴定为一个假定的转录调节因子，并通过序列分析被归类为MerR家族。在红串红球菌中成功地产生了经纯化的SCO 5550，其可用于通过在宽范围（4-35°C）内优化温度来稳定表达重组蛋白。晶体结构分析表明，SCO 5550的二聚化结构域（C-末端结构域）有一个新的折叠，形成一个新的二聚体形状，而DNA结合结构域（N-末端结构域）是非常相似的MerR家族成员。这种新的二聚体形式表明SCO 5550可能定义了一个新的亚家族作为MerR家族的新成员。结合DNA序列分析的SCO 5550使用的配体的基因组系统进化指数富集（gSELEX）和电泳迁移率变动分析（EMSA）表明，SCO 5550调节的直接上游基因sco 5551编码一个推定的蛋白质的表达，可能作为一个转录激活因子。
SCO5550 from the model actinomycete Streptomyces coelicolor A3(2) was identified as a putative transcriptional regulator, and classified into the MerR family by sequence analysis. Recombined SCO5550 was successfully produced in Rhodococcus erythropolis, which can be used to stably express recombinant protein by optimizing the temperature over a wide range (4–35°C). Crystal structure analysis showed that the dimerization domain (C-terminal domain) of SCO5550 has a novel fold and forms a new dimer shape, whereas the DNA-binding domain (N-terminal domain) is very similar to those of MerR family members. Such the new dimer form suggests that SCO5550 may define a new subfamily as a new member of the MerR family. Binding DNA sequence analysis of SCO5550 using the genomic systematic evolution of ligands by exponential enrichment (gSELEX) and electrophoretic mobility shift assay (EMSA) indicated that SCO5550 regulates the expression of the immediately upstream gene sco5551 encoding a putative protein, probably as a transcriptional activator.