Implication of the "4S" polycyclic aromatic hydrocarbon binding protein in the transregulation of rat cytochrome P-450c expression.
Implication of the "4S" polycyclic aromatic hydrocarbon binding protein in the transregulation of rat cytochrome P-450c expression.
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DOI：
10.1021/bi00347a050
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发表时间：
1985-12
期刊：
影响因子：
2.9
通讯作者：
W. Houser;R. Hines;E. Bresnick
中科院分区：
文献类型：
--
作者：
W. Houser;R. Hines;E. Bresnick
通过离子交换、凝胶渗透和疏水作用层析，从大鼠肝细胞浆中纯化了一种与[~3H]苯并[a]芘和其他多环芳烃特异结合的蛋白质，纯化倍数超过6000倍。该结合蛋白不同于其他实验室鉴定的9S结合蛋白。Sephadex G-100凝胶过滤测定斯托克斯半径为2.75 nm。用蔗糖梯度法测得沉淀值为3.3S。研究了该蛋白与大鼠肝脏总DNA以及含有部分大鼠细胞色素P-450c基因的亚克隆的结合能力。在高度严格的条件下，该结合蛋白与含有5‘-上游序列的大鼠细胞色素P-450c基因的几个亚克隆以及部分内含子1以一种特定的饱和方式相互作用。未观察到与该基因编码部分的结合。这些数据表明，“4S”结合蛋白参与了大鼠细胞色素P-450c表达的反式调节。
A protein which specifically binds [3H]benzo[a]pyrene and other polycyclic aromatic hydrocarbons has been purified over 6000-fold from rat hepatic cytosol by using ion-exchange, gel permeation, and hydrophobic interaction chromatography. The binding protein differs from the 9S binding protein characterized in other laboratories. A Stokes radius of 2.75 nm was determined by gel filtration on Sephadex G-100. A sedimentation coefficient of 3.3 S was determined by using sucrose gradient analysis. The ability of this protein to bind total rat liver DNA as well as subclones containing portions of the rat cytochrome P-450c gene was investigated. Under high stringency conditions, this binding protein was found to interact in a specific and saturable manner with several subclones of the rat cytochrome P-450c gene containing 5'-upstream sequences, as well as portions of intron 1. Binding was not observed to the coding portions of the gene. These data implicate the "4S" binding protein in the transregulation of rat cytochrome P-450c expression.