Effects of genital tract inflammation on human immunodeficiency virus type 1 V3 populations in blood and semen
Effects of genital tract inflammation on human immunodeficiency virus type 1 V3 populations in blood and semen
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DOI：
10.1128/jvi.74.19.8946-8952.2000
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发表时间：
2000-10-01
影响因子：
5.4
通讯作者：
Nelson, JAE
中科院分区：
文献类型：
--
作者：
Ping, LH;Cohen, MS;Nelson, JAE
我们已经研究了无细胞的病毒群体在血浆和精浆室感染C亚型人类免疫缺陷病毒1型（HIV-1）的男性使用V3特异性异源双链追踪试验（V3-HTA）。我们研究了两组受试者，他们在马拉维的性传播疾病（STD）诊所以尿道炎的形式（n = 43）或皮肤科诊所（对照组，n = 14）就诊。我们先前已经证明尿道炎的存在与精浆中病毒载量的八倍增加有关（M。S.科恩等人，Lancet 349：1868-1873，1997）。本研究的目的是确定生殖道炎症及其治疗是否会导致无细胞HIV-1群体的遗传不稳定性，在研究开始时的横断面分析中，四分之三的STD和对照受试者血液中有多个V3群体，而60%的STD受试者和79%的对照受试者精液中有多个V3群体。总体而言，当比较样本是否存在亚群时，四分之一的受试者显示血液和精液标本结果不一致。当条带丰度的相对水平差异也被考虑在内时，五分之二的受试者显示出分区之间的不一致。在可检测到多种病毒群体的受试者亚组中，一半搁置了区室之间的不一致性。在研究进入时的该横断面分析中，STD和对照组群之间对于这些区室的比较没有差异。对来自1至4周内的两次单独诊所访视的病毒群体的纵向分析显示，通过香农熵测量的每个V3群体的复杂性在两个时间点在血液和精液中是不同的，这表明血液和精液构成了HIV-1的不同部分。对于接受尿道炎治疗的STD受试者，精浆隔室比血浆隔室更具动态性，在这些受试者的精液中有或没有V3-HTA条带的情况下观察到变化，但这些受试者中只有9%的血液中有V3-HTA条带。然而，变化通常很小，总体而言，我们的结果表明，40%的男性受试者显示精液和血液病毒群体之间的不一致性，并且每个V3群体的复杂性在两个区室之间是不同的。这两个结果都表明，作为体内病毒小生境的精液室是部分独立的。
We have examined cell-free viral populations in the blood plasma and seminal plasma compartments of men infected with subtype C human immunodeficiency virus type 1 (HIV-1) using the V3-specific heteroduplex tracking assay (V3-HTA). We studied two cohorts of subjects who had visited either a sexually transmitted disease (STD) clinic for genital tract inflammation in the form of urethritis (n = 43) or a dermatology clinic (controls, n = 14) in Malawi. We have previously shown that the presence of urethritis Is associated with an eightfold increase in virus load in the seminal plasma compartment (M. S. Cohen ct al., Lancet 349:1868-1873, 1997). The purpose of this study was to determine whether genital tract inflammation and its treatment caused genetic instability in cell-free HIV-1 populations, In a cross-sectional analysis at study entry, three-fourths of the STD and control subjects had multiple V3 populations in their blood while 60% of the STD subjects and 79% of the control subjects had multiple V3 populations in their semen. Overall, one-fourth of an of the subjects show-ed discordance between results with blood and semen specimens when samples were compared for the presence and absence of subpopulations. When differences in the relative levels of abundance of bands were also taken into account, two-fifths of all of the subjects showed discordance between the compartments. Among the subset of subjects in whom multiple virus populations could be detected, half shelved discordance between the compartments. There were no differences between STD and control cohorts for these comparisons of the compartments in this cross-sectional analysis at study entry, Longitudinal analysis of the viral populations from two separate clinic visits over 1 to 4 weeks showed that the complexity of each V3 population as measured by Shannon entropy was different in blood and semen at the two time points, indicating that the blood and semen constitute different compartments for HIV-1. The seminal plasma compartment was more dynamic than the blood plasma compartment for the STD subjects who were treated for urethritis, with changes being noted in the presence or absence of V3-HTA bands in the semen of 29% of these subjects but in the blood of only 9% of these subjects. However, the changes were generally small, Overall, our results suggest that 40% of male subjects show discordance between seminal and blood viral populations and that the complexity of each V3 population was different between the two compartments. Both of these results point to the partial independence of the seminal compartment as a viral niche within the body.