Decreased expression of updated NESG1 in nasopharyngeal carcinoma: its potential role and preliminarily functional mechanism
Decreased expression of updated NESG1 in nasopharyngeal carcinoma: its potential role and preliminarily functional mechanism
复制标题
更新后的NESG1在鼻咽癌中的表达降低：其潜在作用和初步功能机制
DOI：
10.1002/ijc.25595
复制
发表时间：
2011-06-01
影响因子：
6.4
通讯作者：
Fang, Weiyi
中科院分区：
文献类型：
--
作者：
Liu, Zhen;Li, Xin;Fang, Weiyi
人NESG 1（CCDC 19）基因最初是在我们实验室从人鼻咽组织中分离的。然而，该基因的生物学和临床意义在很大程度上仍然未知。本报告发现原提交的人NESG 1基因序列中有两处错误，并在NCBI数据库（登录号：NM_012337. 2）中对NESG 1开放阅读框架序列（登录号：NM_012337. 1）进行了修订和更新。针对NESG 1的修订序列产生的抗体在人鼻咽组织中检测到66 kD的单一条带。NESG 1转录本在鼻咽上皮中特异性表达。与正常鼻咽组织相比，鼻咽癌（NPC）组织和细胞系中NESG 1转录本和蛋白的表达下调或缺失。NESG 1蛋白在低级别鼻咽癌组织中的表达明显高于高级别鼻咽癌组织。在NESG 1阴性的5- 8 F细胞中诱导NESG 1的表达不仅显著降低细胞增殖、G1-S期转变，而且显著抑制细胞的迁移和侵袭能力以及体内成瘤性。此外，NESG 1还显著调节细胞周期调节因子CCNA 1和p21的表达。我们的研究结果首次证明NESG 1可能通过抑制鼻咽癌细胞的增殖、侵袭和迁移而发挥抑癌作用。
Human NESG1 (CCDC19) gene was originally isolated in our laboratory from human nasopharynx tissue. However, the biological and clinical significances of this gene remain largely unknown. In this report, two errors in the originally submitted sequence of human NESG1 gene were found, and the open reading frame sequence of NESG1 (Accession number: NM_012337.1) was revised and updated in the NCBI database (Accession number: NM_012337.2). The antibody raised against the revised sequence of NESG1 detected a single band of 66 kD in human nasopharynx tissues. NESG1 transcripts were specifically expressed in the nasopharynx epithelium. Expression of NESG1 transcripts and protein was downregulated or absent in nasopharyngeal carcinoma (NPC) tissues and cell lines in comparison to that in the normal nasopharynx tissues. The levels of NESG1 protein were significantly greater in the low-grade NPC tissues than that in the high-grade NPC tissues. Induced expression of NESG1 in otherwise NESG1-negative 5-8F cells not only significantly decreased cell proliferation, G1-S phase transition, but also markedly inhibited the ability of cell migration and invasion as well as in vivo tumorigenesis. Furthermore, NESG1 also significantly regulated the expression of cell cycle regulator CCNA1 and p21. Our findings first provided evidence that NESG1 may act as a tumor suppressor by inhibiting cell proliferation, invasion and migration of NPC cells.