Hyperlipidemic mice as a model for a real-time in vivo detection of atherosclerosis by gold nanorods-based diffusion reflection technique
Hyperlipidemic mice as a model for a real-time in vivo detection of atherosclerosis by gold nanorods-based diffusion reflection technique
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DOI：
10.1002/jbio.201800218
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发表时间：
2019-01-01
影响因子：
2.8
通讯作者：
Fixler, Dror
中科院分区：
文献类型：
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作者：
Chalcraborty, Ruchira;Ankri, Rinat;Fixler, Dror
动脉粥样硬化(AS)是心血管疾病发病率和死亡率的主要原因，需要及早发现，以治疗和预防致命事件。在这里，我们首次应用了金纳米棒(GNRs)辅助扩散反射(DR)，这是一种非侵入性技术，用于在高脂饮食诱导的C57BL小鼠模型中检测AS，这与人类AS的表现相似。DR只是检测由于AS斑块中聚集的GNRs而导致的组织光反射轮廓的变化，并能够清楚地检测到这些高脂血症小鼠颈动脉和股动脉中的AS损害。注射GNRs后24小时，DR对AS的检测效率最高。此外，DR方法的敏感性远高于CT，可与体外高分辨率CT相媲美。我们的结果有力地表明，DR方法可以敏感和特异地发现早期动脉粥样硬化病变。
Atherosclerosis (AS), the leading cause of morbidity and mortality in cardiovascular disease, needs an early detection for treatment and prevention of fatal events. Here, for the first time, we applied gold nanorods (GNRs)-assisted diffusion reflection (DR), a noninvasive technique for in vivo detection of AS in a high-fat-diet-induced c57bl mouse model, which resembles the manifestation of AS in humans. DR simply detects the change in light reflection profile of tissue due to the accumulation of GNRs in the AS plaques and enables clear detection of AS lesions in carotid and femoral arteries of these hyperlipidemic mice. After 24 hours post-GNRs injection, DR showed the highest efficiency of AS detection. Moreover, the sensitivity of the DR method is much higher than computed tomography (CT) and is comparable to ex vivo high-resolution CT. Our results strongly suggest that the DR method can detect early atherosclerotic lesions in a sensitive and specific manner.