Myocardial metabolism in chronic reperfusion after nontransmural infarction in pig hearts.
Myocardial metabolism in chronic reperfusion after nontransmural infarction in pig hearts.
复制标题
猪心脏非透壁性梗死后慢性再灌注中的心肌代谢。
DOI：
10.1152/ajpheart.1993.265.5.h1614
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
Woldegiorgis,G
中科院分区：
文献类型：
--
作者：
Liedtke,AJ;Renstrom,B;Nellis,SH;Subramanian,R;Woldegiorgis,G
这些研究的目的是评价诱导内膜下梗死后猪4天再灌注模型中的代谢行为。两组猪[假手术组和干预（Int）组，n = 7），每组分别为10个心脏]分别在4天内进行两次外科手术。在Int组中，首次手术时，左前降支（LAD）循环中的冠状动脉血流部分受限（60%）60分钟，然后再灌注。在第二次手术时，对两组的LAD心肌进行体外有氧灌注（5.9 +/- 0.2 ml.min-1.g dry wt-1）60 min，并通过[U-14 C]-棕榈酸酯和[5- 3 H]葡萄糖平衡标记输注，以估计脂肪酸氧化和外源性葡萄糖利用。在体外灌注过程中，尽管线粒体ATP再合成受到轻微损害，但两组之间的局部心肌缩短和耗氧量相当（减少26%，P < 0.071），线粒体呼吸显著下降（呼吸控制率降低45%，P < 0.008;状态3呼吸降低41%，P < 0.032）。在假心脏中，由14 CO2产生描述的脂肪酸氧化为34.00 +/- 4.72 mumol.h-1.g干重-1（灌注30-60分钟的平均值），但在Int心脏中降低（48%，P < 0.004）。这种脂肪酸利用率的降低可以部分地由观察到的线粒体膜转运酶肉毒碱棕榈酰转移酶活性的下降来解释。（250字处删节）
The purpose of these studies was to evaluate metabolic behavior in a 4-day reperfusion model in pigs after induction of subendocardial infarction. Two groups of swine [sham and intervention (Int) groups, n = 7) and 10 hearts per group, respectively] were prepared comparably with two surgical procedures separated over 4 days. In the Int group at the time of the first surgery, coronary flow in the left anterior descending (LAD) circulation was partially restricted (by 60%) for 60 min and was then reperfused. LAD myocardium at the time of the second surgery in both groups was extracorporeally perfused aerobically (5.9 +/- 0.2 ml.min-1.g dry wt-1) for 60 min and infused by equilibrium labeling with [U-14C]-palmitate and [5-3H]glucose to estimate fatty acid oxidation and exogenous glucose utilization. During extracorporeal perfusion, regional myocardial shortening and oxygen consumption were comparable between groups despite a marginal impairment in ATP resynthesis by mitochondria (26% decrease, P < 0.071) in Int hearts and a significant decline in mitochondrial respiration (45% decrease in respiratory control rate, P < 0.008; and 41% decrease in state 3 respiration, P < 0.032) as compared with sham hearts. Fatty acid oxidation described by 14CO2 production was 34.00 +/- 4.72 mumol.h-1.g dry wt-1 (averaged from 30-60 min of perfusion) in sham hearts but was decreased (by 48%, P < 0.004) in Int hearts. This reduction in fatty acid utilization may in part be explained by declines in the observed activity of the mitochondrial membrane transporter enzyme, carnitine palmitoyltransferase.(ABSTRACT TRUNCATED AT 250 WORDS)