Photo Control of Protein Function Using Photoactive Yellow Protein.
Photo Control of Protein Function Using Photoactive Yellow Protein.
复制标题
使用光活性黄色蛋白对蛋白质功能进行光控制。
DOI：
10.1007/978-1-4939-3512-3_6
复制
发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Woolley,GAndrew
中科院分区：
文献类型：
--
作者：
Reis,JakebM;Woolley,GAndrew
光开关蛋白正成为以高空间和时间精度操纵细胞过程的越来越常见的工具。光敏黄蛋白（PYP）是一种小的水溶性蛋白质，其经历蓝光诱导的构象变化。它可以作为设计新工具来操纵生物过程的支架，但相对于其他蛋白质支架，它提出了一些技术挑战。在这里，我们提出了实用的信息，如何克服这些，包括如何合成PYP发色团，如何表达和纯化PYP，以及如何筛选所需的活动。
Photoswitchable proteins are becoming increasingly common tools for manipulating cellular processes with high spatial and temporal precision. Photoactive yellow protein (PYP) is a small, water-soluble protein that undergoes a blue light induced change in conformation. It can serve as a scaffold for designing new tools to manipulate biological processes, but with respect to other protein scaffolds it presents some technical challenges. Here, we present practical information on how to overcome these, including how to synthesize the PYP chromophore, how to express and purify PYP, and how to screen for desired activity.