Evaluation of the effect of green tea and its constituents on embryo development in a zebrafish model
Evaluation of the effect of green tea and its constituents on embryo development in a zebrafish model
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评估绿茶及其成分对斑马鱼模型胚胎发育的影响。
DOI：
10.1002/jat.4380
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发表时间：
2022-08-28
影响因子：
3.3
通讯作者：
Chen，Dongyan
中科院分区：
文献类型：
--
作者：
Zhang，Qiuping;Gao，Qian;Chen，Dongyan
作为最受欢迎的饮料之一，绿色茶因其对人体健康的有益作用而引起了人们的极大兴趣。然而，绿色茶的毒性及其潜在机制仍然知之甚少。在这里，我们评估了绿色茶及其成分对斑马鱼胚胎发育的影响。形态学结果表明，0.1%和0.2%的绿色茶增加了死亡率，推迟了原肠胚形成的外延，缩短了体长。绿色茶改变了dlx 3、cstlb、myod和papc的表达模式，降低了wnt 5和wnt 11的表达水平，表明绿色茶通过下调wnt 5和wnt 11干扰了会聚和伸展运动。0.1%和0.2%绿色茶处理的胚胎背腹基因表达增加，而wnt 8及其靶基因voxandvenin表达减少，表明绿色茶可能通过抑制wnt 8信号通路影响背腹分化。此外，绿色茶可以通过在原肠胚形成过程中破坏F-肌动蛋白组织或去除植物性蛋黄中的F-肌动蛋白来抑制外延进展。然而，单独暴露于五种儿茶素和没食子酸没有引起畸形。成分混合后对胚胎的影响与绿色茶液相似，如眼睛和头部变小，体长变短，心率变慢，表明绿色茶液对胚胎发育的影响主要是由于绿色茶液中多种成分的综合作用。
As one of the most popular beverages, green tea has attracted much interest for its beneficial effects on human health. However, the toxicity of green tea and its underlying mechanism are still poorly understood. Here, we evaluated the effect of green tea and its constituents on development by exposing zebrafish embryos to them. Morphologic results demonstrated that 0.1% and 0.2% green tea increased mortality, delayed epiboly of gastrulation, and shortened body length. Green tea altered the expression pattern ofdlx3,cstlb,myod, andpapcand decreased the expression levels ofwnt5andwnt11, suggesting that green tea disturbed convergence and extension movement through the downregulation ofwnt5andwnt11. The increased expression of the dorsal genechordinand reduced expression ofwnt8and its target genesvoxandventin embryos exposed to 0.1% and 0.2% green tea indicated that green tea could affect dorsoventral differentiation by inhibiting the wnt8 signaling pathway. Additionally, green tea could inhibit epiboly progression by disrupting F‐actin organization or removing F‐actin in vegetal yolks during gastrulation. However, no malformation was caused by exposure to the five catechins and gallic acid individually. The mixture of constituents showed a similar effect to green tea solution on the embryos, such as smaller eyes and head, shorter body length, and slower heart rate, which indicated that the effect of green tea solution on embryo development was mainly due to the comprehensive effect of multiple components in the green tea solution.