A Phytophthora effector promotes homodimerization of host transcription factor StKNOX3 to enhance susceptibility.
A Phytophthora effector promotes homodimerization of host transcription factor StKNOX3 to enhance susceptibility.
复制标题
疫霉效应子促进宿主转录因子 StKNOX3 的同二聚化以增强敏感性。
DOI：
10.1093/jxb/erac308
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发表时间：
2022
影响因子：
6.9
通讯作者：
Zhou J
中科院分区：
文献类型：
--
作者：
Zhou J
Oomycete pathogens secrete hundreds of cytoplasmic RxLR effectors to modulate host immunity by targeting diverse plant proteins. Revealing how effectors manipulate host proteins is pivotal to understanding infection processes and to developing new strategies to control plant disease. Here we show that thePhytophthora infestansRxLR effector Pi22798 interacts in the nucleus with a potato class II knotted-like homeobox (KNOX) transcription factor, StKNOX3. Silencing the orthologNbKNOX3inNicotiana benthamianareduces host colonization byP. infestans, whereas transient and stable overexpression ofStKNOX3enhances infection. StKNOX3 forms a homodimer which is dependent on its KNOX II domain. The KNOX II domain is also essential for Pi22798 interaction and for StKNOX3 to enhanceP. infestanscolonization, indicating that StKNOX3 homodimerization contributes to susceptibility. However, critically, the effector Pi22798 promotes StKNOX3 homodimerization, rather than heterodimerization to another KNOX transcription factor StKNOX7. These results demonstrate that the oomycete effector Pi22798 increases pathogenicity by promoting homodimerization specifically of StKNOX3 to enhance susceptibility.
