Insights into electrosensory organ development, physiology and evolution from a lateral line-enriched transcriptome
Insights into electrosensory organ development, physiology and evolution from a lateral line-enriched transcriptome
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DOI：
10.7554/elife.24197
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发表时间：
2017-03-27
期刊：
影响因子：
7.7
通讯作者：
Baker, Clare V. H.
中科院分区：
文献类型：
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作者：
Modrell, Melinda S.;Lyne, Mike;Baker, Clare V. H.
Anamniote的侧线系统包括机械感觉神经瘤和电沉积器官，是研究不同器官和细胞类型的发育和进化多样化的有用模型。斑马鱼神经瘤发育越来越充分了解，但是斑马鱼和爪蟾都不是电的，我们对截肢器官发育的分子理解是基本的。我们已经使用RNA-seq从晚期paddlefish（Polyodon Spathula）产生了横向线的基因列。对子集的验证揭示了发展对毛细胞发育至关重要的转录因子基因的截肢器官的表达，以及在毛细胞色带突触下谷氨酸释放必不可少的基因，这表明非teleost电动器和毛细胞之间的近距离发育，生理和进化连接。我们确定了隔离器官特异性的隆起转录因子，以及电压感应L型CAV通道的候选和矫正从滑冰（软骨鱼）安排的隔离器机器生理学预测的KV通道。总体而言，我们的结果阐明了隔膜器官的发育，生理和进化。
The anamniote lateral line system, comprising mechanosensory neuromasts and electrosensory ampullary organs, is a useful model for investigating the developmental and evolutionary diversification of different organs and cell types. Zebrafish neuromast development is increasingly well understood, but neither zebrafish nor Xenopus is electroreceptive and our molecular understanding of ampullary organ development is rudimentary. We have used RNA-seq to generate a lateral line-enriched gene-set from late-larval paddlefish (Polyodon spathula). Validation of a subset reveals expression in developing ampullary organs of transcription factor genes critical for hair cell development, and genes essential for glutamate release at hair cell ribbon synapses, suggesting close developmental, physiological and evolutionary links between non-teleost electroreceptors and hair cells. We identify an ampullary organ-specific proneural transcription factor, and candidates for the voltage-sensing L-type Cav channel and rectifying Kv channel predicted from skate (cartilaginous fish) ampullary organ electrophysiology. Overall, our results illuminate ampullary organ development, physiology and evolution.