A survey of genes expressed in adults of the human hookworm, Necator americanus
A survey of genes expressed in adults of the human hookworm, Necator americanus
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DOI：
10.1017/s0031182099005375
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发表时间：
2000-02-01
期刊：
影响因子：
2.4
通讯作者：
Blaxter, M
中科院分区：
文献类型：
--
作者：
Daub, J;Loukas, A;Blaxter, M
钩虫是一种寄生在肠道的吸血线虫，感染数亿人，特别是在热带地区。作为旨在确定人类寄生线虫新药物靶点和候选疫苗计划的一部分，通过表达序列标签方法对人类钩虫美洲钩虫成虫中表达的基因进行了调查。总共鉴定出 161 个新的钩虫基因。对于其中的大多数，可以通过同源性来指定功能。该数据集包括蛋白酶、蛋白酶抑制剂、脂质结合蛋白、C 型凝集素、抗菌因子、珠蛋白和其他从药物或疫苗开发角度来看感兴趣的基因。鉴定出三种不同类别的小分泌蛋白，它们可能参与宿主-寄生虫相互作用，包括潜在的钾通道阻断肽。三分之一的基因是新的。其中包括高表达的分泌型（糖）蛋白，它们可能是这些重要病原体的排泄分泌产物的一部分。特别令人感兴趣的是一个由 9 个基因组成的家族，它们与免疫调节蛋白、中性粒细胞抑制因子相似，可能在为这种成功的寄生虫建立免疫受损的生态位中发挥作用。
Hookworms are gut-dwelling, blood-feeding nematodes that infect hundreds of millions of people, particularly in the tropics. As part of a program aiming to define novel drug targets and vaccine candidates for human parasitic nematodes, genes expressed in adults of the human hookworm Necator americanus were surveyed by the expressed sequence tag approach. In total 161 new hookworm genes were identified. For the majority of these, a function could be assigned by homology. The dataset includes proteases, protease inhibitors, a lipid binding protein, C-type lectins, an anti-bacterial factor, globins and other genes of interest from a drug or vaccine development viewpoint. Three different classes of small, secreted proteins were identified that may be involved in the host-parasite interaction, including potential potassium channel blocking peptides. One third of the genes were novel. These included highly expressed, secreted (glyco)proteins which may be part of the excretory-secretory products of these important pathogens. Of particular interest are a family of 9 genes with similarity to the immunomodulatory protein, neutrophil inhibitory factor, that may play a role in establishing an immunocompromised niche for this successful parasite.