Fluorescence ratiometric DNA detection by peptide nucleic acid-pyrene binary probes
Fluorescence ratiometric DNA detection by peptide nucleic acid-pyrene binary probes
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DOI：
10.1016/j.bmcl.2022.128838
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发表时间：
2022-06-04
影响因子：
2.7
通讯作者：
Kitamatsu，Mizuki
中科院分区：
文献类型：
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作者：
Ishii，Koki;Tsuchitani，Sakura;Kitamatsu，Mizuki
我们建立了一种用芘修饰的两种肽核酸（PNAs）的准分子荧光检测DNA的方法。两种PNA-Pyr探针通过固相肽合成制备，我们评估了探针与DNA混合物的荧光。结果显示，与互补DNA形成杂交的两个PNA-Pyr探针产生的准分子荧光，当探针与DNA以1:1:1的比例杂交时，两个探针显示出最大的准分子/单体比，表明PNA-Pyr探针可以检测到目标DNA。此外，我们调整了DNA上形成的两个PNA-Pyr杂交种之间的空间排列，以促进最佳的准分子形成。因此，通过在形成的两个杂化体之间间隔两个核碱基，并在PNA-Pyr探针的一侧PNA和Pyr之间进一步插入六亚甲基连接体（C6），可以实现最佳的准分子形成。
We developed a method for detecting DNA by excimer fluorescence from two peptide nucleic acids (PNAs) modified with a pyrene (Pyr). The two PNA-Pyr probes were prepared by solid-phase peptide synthesis, and we assessed fluorescence from the mixture of probes with DNA. From the results, excimer fluorescence derived from the two PNA-Pyr probes forming hybrids with the complementary DNA was observed, and the two probes showed the maximum excimer/monomer ratio when the probes and DNA were hybridized at a 1:1:1 ratio, indicating that the PNA-Pyr probes can detect target DNA. Furthermore, we adjusted the spatial arrangement between the two PNA-Pyr hybrids formed on the DNA to promote optimal excimer formation. As a result, optimal excimer formation was achieved by spacing the two nucleobases between the formed two hybrids and further inserting a hexamethylene linker (C6) between the PNA and Pyr of the PNA-Pyr probe on one side.