Effect of nanoparticle-rich diesel exhaust on testosterone biosynthesis in adult male mice
Effect of nanoparticle-rich diesel exhaust on testosterone biosynthesis in adult male mice
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DOI：
10.3109/08958378.2012.702140
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发表时间：
2012-08-01
影响因子：
2.1
通讯作者：
Taya, Kazuyoshi
中科院分区：
文献类型：
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作者：
Li, ChunMei;Li, Xuezheng;Taya, Kazuyoshi
研究了富含纳米柴油的尾气(NR-DE)对小鼠睾丸功能的影响及其与睾酮基因表达生物合成的相关因素。将雄性C57BL/JCL小鼠暴露于清洁空气、低剂量NR-DE(低NR-DE)、大剂量NR-DE(高NR-DE)或过滤柴油尾气(F-DE)中8周。我们发现，暴露于高NR-DE组的小鼠的睾酮水平显著高于对照组和F-DE组。为确定天然DE对睾丸睾酮生成的影响，将暴露于NR-DE、F-DE或清洁空气中8周的雄性小鼠分离的间质细胞与人绒毛膜促性腺激素(hCG；0.1IU/mL)孵育4h，测定培养液中睾酮的浓度。高NR-DE染毒组无论有无hCG，睾酮生成量均显著增加，而F-DE染毒组无论有无hCG，睾酮生成量均显著降低。此外，在成年雄性小鼠的睾丸中还检测到了与睾丸胆固醇合成相关的几个基因：HMG-CoA、LDL-R、SR-B1、PBR和P450scc、P450 17α和17β-HSD。结果表明，较高的NR-DE暴露显著增加了这些基因的表达。然而，F-DE暴露组的水平恢复到对照组的水平，这意味着DE中的纳米颗粒对观察到的生殖毒性起到了作用。我们的结论是，NR-DE暴露对睾酮生物合成的促进可能是通过增加睾丸睾酮生物合成的酶来调节的。
The effect of nanoparticle-rich diesel exhaust (NR-DE) on the testicular function and factors related with the biosynthesis of testosterone gene expression were investigated in mice. Male C57BL/Jcl mice were exposed to clean air, low-dose NR-DE (Low NR-DE), high-dose NR-DE (High NR-DE) or filtered diesel exhaust (F-DE) for 8 weeks. We found that the mice exposed to High NR-DE had significantly higher testosterone levels than those in the control and F-DE groups. To determine the effects of NR-DE on testicular testosterone production, interstitial cells dissected from the male mice which were exposed to NR-DE, F-DE, or clean air for 8 weeks were incubated with or without human chorionic gonadotropin (hCG; 0.1 IU/mL) for 4 h. The concentrations of testosterone in the culture media were measured. The testosterone production was significantly increased in with or without hCG of High NR-DE exposed group, and significantly decreased in both with or without hCG of F-DE exposed groups. Moreover, several genes, which is associated with testicular cholesterol synthesis, HMG-CoA, LDL-R, SR-B1, PBR, and P450scc, P450 17 alpha, and 17 beta-HSD were determined in the testis of adult male mice. The results showed High NR-DE exposure significantly increased the expression of these genes. Whereas, the levels in the F-DE exposure group returned to those in the control group, implicating that the nanoparticles in DE contribute to the observed reproductive toxicity. We conclude that enhancement of testosterone biosynthesis by NR-DE exposure may be regulated by increasing testicular enzymes of testosterone biosynthesis.