Absence of bovine leukemia virus proviral DNA in Japanese human blood cell lines and human cancer cell lines
Absence of bovine leukemia virus proviral DNA in Japanese human blood cell lines and human cancer cell lines
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日本人血细胞系和人癌细胞系中不存在牛白血病病毒前病毒 DNA
DOI：
10.1007/s00705-019-04474-9
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发表时间：
2019
影响因子：
2.7
通讯作者：
Yoko Aida.
中科院分区：
文献类型：
--
作者：
Susumu Saito;Yuri Kitamura-Muramatsu; Fumiko Komine;Meripet Polat;Shin-nosuke Takeshima;Masami Takei;Yoko Aida.
牛白血病病毒(BLV)在世界范围内感染牛，并引起牛的B细胞淋巴瘤。BLV已在人类乳腺癌、肺癌和血液中被发现，但BLV与人类癌症的联系存在争议。在这项研究中，我们使用了一种新的高灵敏度的聚合酶链式反应方法，用不同于以往乳腺癌BLV前病毒研究的LTR型引物，甚至可以扩增出一个拷贝的BLV，从而调查了145个日本血细胞系和54个人类癌细胞系中BLV的存在。所有样本都被发现BLV前病毒呈阴性，这表明BLV不太可能感染人类。
Bovine leukemia virus (BLV) infects cattle worldwide and causes B-cell lymphoma in cattle. BLV has been identified in human breast and lung cancer and in blood, but the association of BLV and human cancer is controversial. In this study, we investigated the existence of BLV in 145 Japanese human blood cell lines and 54 human cancer cell lines, using a new highly sensitive PCR assay that can amplify even one copy of BLV using LTR primers different from those in previous studies on BLV provirus in breast cancer. All samples were found negative for BLV provirus, suggesting that BLV is unlikely to infect humans.