Molecular mechanism of the suppression of larval skeleton by polycyclic aromatic hydrocarbons in early development of sea urchin Hemicentrotus pulcherrimus.
Molecular mechanism of the suppression of larval skeleton by polycyclic aromatic hydrocarbons in early development of sea urchin Hemicentrotus pulcherrimus.
复制标题
海胆Hemicentrotus pulcherrimus早期发育过程中多环芳烃抑制幼虫骨骼的分子机制。
DOI：
10.1007/s12562-018-1245-y
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发表时间：
2018
影响因子：
1.9
通讯作者：
N.
中科院分区：
文献类型：
--
作者：
Sekiguchi;T.;Yachiguchi;K.;Kiyomoto;M.;Ogiso;S.;Wada;S.;Tabuchi;Y.;Hong;C.-S.;Srivastav;A.K.;Archer;S.;Pointing;S.B.;Hayakawa;K. and Suzuki;N.
苯并[a]蒽（BaA）等多环芳烃是水环境中的优先污染物。我们以前的研究表明，BaA及其代谢产物4-单羟基BaA（4-OHBaA）抑制海胆Hemicentrotus pulcherrimus幼虫的骨骼发生。在这里，我们报告的研究，以阐明由BaA和4-OHBaA引起的骨骼发育抑制的目标。首先，我们进行了一个体外实验，使用分离的微粒，从而产生幼虫的骨骼间充质。BaA和4-OHBaA对幼虫骨骼发育无明显抑制作用，提示BaA和4-OHBaA对幼虫骨骼发育有间接影响。接下来，我们使用胚胎分析了它们在体内的影响。BaA和4-OHBaA处理抑制了外胚层中表达并诱导骨针形成的血管内皮生长因子（VEGF）。这些化学物质还抑制了硫酸乙酰肝素6-O-硫酸酯酶（Sulf）的表达，该酶被称为VEGF信号传导调节剂。因此，我们认为BaA和4-OHBaA通过VEGF信号通路影响幼虫骨骼发育。此外，我们发现，内胚层标记物Endo 16 mRNA的表达在BaA和4-OHBaA暴露后降低，表明这些化学物质影响内胚层功能以及骨骼生成。本研究表明，BaA和4-OHBaA对H.一品红
Polycyclic aromatic hydrocarbons including benz[a]anthracene (BaA) are priority pollutants in the aquatic environment. Our previous study revealed that BaA and its metabolite, 4-monohydroxylated BaA (4-OHBaA) inhibit larval skeletogenesis in the sea urchinHemicentrotus pulcherrimus. Here we report studies to elucidate the target of skeletogenesis inhibition elicited by BaA and 4-OHBaA. First, we performed an in vitro experiment using isolated micromeres which give rise to the larval skeletogenic mesenchyme. However, skeletogenesis was not repressed by BaA and 4-OHBaA, implying that these chemicals indirectly influence on the formation of larval skeleton. Next, we analyzed their influence in vivo using embryos. Vascular endothelial growth factor (VEGF) that is expressed in the ectoderm and induces spicule formation was inhibited by BaA and 4-OHBaA treatment. These chemicals also suppressed the expression of the heparan sulfate 6-Oendosulfatase (Sulf) known as a VEGF signaling modulator. We, therefore, propose that BaA and 4-OHBaA effects on larval skeletogenesis via VEGF signaling. Furthermore, we showed that the expression of Endo16 mRNA, an endodermal marker, decreased after BaA and 4-OHBaA exposure, suggesting that these chemicals affect endodermal function together with skeletogenesis. This study demonstrates that BaA and 4-OHBaA exert multiple detrimental effects on the development ofH. pulcherrimus.