LATE STAGES IN THE BIOSYNTHESIS OF AUCUBIN IN SCROPHULARIA
LATE STAGES IN THE BIOSYNTHESIS OF AUCUBIN IN SCROPHULARIA
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DOI：
10.1016/0031-9422(93)85028-p
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发表时间：
1993-07-01
期刊：
影响因子：
3.8
通讯作者：
KNUDSEN, TB
中科院分区：
文献类型：
--
作者：
DAMTOFT, S;JENSEN, SR;KNUDSEN, TB
氘标记的8-表-脱氧落甘酸、脱氧脂苷酸、京尼平苷酸样品。和bartsioside（6-deoxyaucubin）都能很好地掺入到玄参中的桃叶珊瑚苷中，而标记的6，10-dideoxyaucubin则没有明显的掺入。In S.在总状花序中，8-表-脱氧马钱酸高掺入桃叶珊瑚苷和哈巴苷中，而8-表-马钱酸不掺入。用标记的8-epi-deoxyloganic酸和未标记的京尼平苷酸或Scandoside进行的稀释实验表明，京尼平苷酸是桃叶珊瑚苷生物合成的中间体，而Scandoside显然不是。从8-epi-deoxyloganic酸到桃叶珊瑚苷的途径因此已经建立，表明脱羧作用发生在京尼平苷酸阶段。
Deuterium-labelled samples of 8-epi-deoxyloganic acid, deoxygeniposidic acid, geniposidic acid. and bartsioside (6-deoxyaucubin) all gave high incorporations into aucubin in Scrophularia umbrosa whereas labelled 6,10-dideoxyaucubin did not give a significant incorporation. In S. racemosa, 8-epi-deoxyloganic acid gave a high incorporation into aucubin and harpagide while 8-epi-loganic acid was not incorporated. Dilution experiments with labelled 8-epi-deoxyloganic acid and unlabelled geniposidic acid or scandoside showed that geniposidic acid is an intermediate in the biosynthesis of aucubin whereas scandoside apparently is not. The pathway from 8-epi-deoxyloganic acid to aucubin has thus been established, showing that decarboxylation takes place at the geniposidic acid stage.