Interaction of leech neurons with topographical gratings: comparison with rodent and human neuronal lines and primary cells
Interaction of leech neurons with topographical gratings: comparison with rodent and human neuronal lines and primary cells
复制标题
水蛭神经元与地形光栅的相互作用：与啮齿动物和人类神经元系和原代细胞的比较
DOI：
10.1098/rsfs.2013.0047
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发表时间：
2014
期刊：
影响因子：
4.4
通讯作者：
M. Cecchini
中科院分区：
文献类型：
--
作者：
I. Tonazzini;M. Pellegrini;M. Pellegrino;M. Cecchini
控制和改善神经细胞的迁移和突起生长是组织工程应用和人工神经元界面开发的关键要素。为此，一种很有前途的方法是利用纳米/微结构表面，这已经被证明能够调节神经元的分化、极性、迁移和轴突方向。在这里，我们研究了水蚤神经元在塑料格栅(GRs；由交替的沟槽和脊线组成的各向异性地形图)上的轴突接触引导。通过高分辨率显微镜，我们定量评估了微管蛋白细胞骨架组织和细胞形态以及轴突和生长锥发育的变化。水蚤神经元在GRs上的地形图阅读过程是由丝状伪足介导的，并且对4-µm周期的GRs的反应比对较小周期的GRs更敏感。最后，将水蛭神经元在GRs上的行为与其他几种神经细胞进行了比较和验证，从小鼠分化的胚胎干细胞和原代海马神经元到分化的人类神经母细胞瘤细胞。
Controlling and improving neuronal cell migration and neurite outgrowth are critical elements of tissue engineering applications and development of artificial neuronal interfaces. To this end, a promising approach exploits nano/microstructured surfaces, which have been demonstrated to be capable of tuning neuronal differentiation, polarity, migration and neurite orientation. Here, we investigate the neurite contact guidance of leech neurons on plastic gratings (GRs; anisotropic topographies composed of alternating lines of grooves and ridges). By high-resolution microscopy, we quantitatively evaluate the changes in tubulin cytoskeleton organization and cell morphology and in the neurite and growth cone development. The topography-reading process of leech neurons on GRs is mediated by filopodia and is more responsive to 4-µm-period GRs than to smaller period GRs. Leech neuron behaviour on GRs is finally compared and validated with several other neuronal cells, from murine differentiated embryonic stem cells and primary hippocampal neurons to differentiated human neuroblastoma cells.