The nitrate-inducible NAC transcription factor NAC056 controls nitrate assimilation and promotes lateral root growth in Arabidopsis thaliana
The nitrate-inducible NAC transcription factor NAC056 controls nitrate assimilation and promotes lateral root growth in Arabidopsis thaliana
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DOI：
10.1371/journal
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发表时间：
2022
期刊：
影响因子：
4.5
通讯作者：
Weiming Cai
中科院分区：
文献类型：
--
作者：
Peipei Xu;Wei Ma;Jinbo Hu;Weiming Cai
Nitrate can affect many aspects of plant growth and development, such as promoting root.growth and inhibiting the synthesis of secondary metabolites. However, the mechanisms.underlying such effects and how plants can integrate nitrate signals and root growth needs.further exploration. Here, we identified a nitrate-inducible NAC family transcription factor.(TF) NAC056 which promoted both nitrate assimilation and root growth in Arabidopsis..NAC056 is a nuclear-localized transcription activator, which is predominantly expressed in.the root system and hypocotyl. Using the yeast one-hybrid assay, we identified the.NAC056-specific binding sequence (NAC56BM), T [T/G/A] NCTTG. We further showed that.the nac056 mutant compromised root growth. NAC056 overexpression promotes LR Initiation. and nitrate deficiency tolerance. Using RNA sequencing analysis and in vitro biochemical. experiment, we found NAC056 regulated the expression of genes required for NO3.−.assimilation, directly targeting the key nitrate assimilation gene NIA1. In addition, mutation.of NIA1 suppresses LR development and nitrate deficiency tolerance in the 35S::NAC056.transgenic plants. Therefore, NAC056 mediates the response of plants to environmental.nitrate signals to promote root growth in Arabidopsis.
