IDENTIFICATION AND CHARACTERIZATION OF DINUCLEOTIDE REPEAT (CA)N MARKERS FOR GENETIC-MAPPING IN DOG
IDENTIFICATION AND CHARACTERIZATION OF DINUCLEOTIDE REPEAT (CA)N MARKERS FOR GENETIC-MAPPING IN DOG
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DOI：
10.1006/geno.1993.1160
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发表时间：
1993-04-01
期刊：
影响因子：
4.4
通讯作者：
RINE, J
中科院分区：
文献类型：
--
作者：
OSTRANDER, EA;SPRAGUE, GF;RINE, J
犬基因组的大量简单序列重复（SSR）多态性已被分离和表征。通过常规杂交方法筛选初级文库以及筛选富集标记选择文库，分离出大量含有（CA）nrepeat的基因组克隆。101个克隆的序列表明，狗基因组中（CA）nrepeat的大小和复杂性与人类基因组中这些标记相似。对这些标记的代表性子集的详细分析显示，大多数标记具有中度到高度多态性，33%的标记的PIC值超过0.70。在这些研究中观察到较高的PIC值与含有较长（CA）nrepeat的标记之间的关联，正如先前在人类基因组中的类似标记所指出的那样。提供了标记每个特征标记的引物序列列表，并建议了犬基因组的综合命名系统。
A large block of simple sequence repeat (SSR) polymorphisms for the dog genome has been isolated and characterized. Screening of primary libraries by conventional hybridization methods as well as by screening of enriched marker-selected libraries led to the isolation of a large number of genomic clones that contained (CA)nrepeats. The sequences of 101 clones showed that the size and complexity of (CA)nrepeats in the dog genome were similar to those reported for these markers in the human genome. Detailed analysis of a representative subset of these markers revealed that most markers were moderately to highly polymorphic, with PIC values exceeding 0.70 for 33% of the markers tested. An association between higher PIC values and markers containing longer (CA)nrepeats was observed in these studies, as previously noted for similar markers in the human genome. A list of primer sequences that tag each characterized marker is provided, and a comprehensive system of nomenclature for the dog genome is suggested.