Histopathological Evaluation of Skeletal Muscle with Specific Reference to Mouse Models of Muscular Dystrophy.
Histopathological Evaluation of Skeletal Muscle with Specific Reference to Mouse Models of Muscular Dystrophy.
复制标题
骨骼肌的组织病理学评估，特别参考肌营养不良小鼠模型。
DOI：
10.1002/cpmo.19
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发表时间：
2016
影响因子：
--
通讯作者：
Terry RL
中科院分区：
文献类型：
--
作者：
Terry RL
肌营养不良症是一组不同的退行性疾病，有许多小鼠模型可供使用。这些模型经常用于评估潜在的治疗干预措施，多块肌肉的组织学评价是该评估的重要部分。组织学评估是特别有用的，当结合测试肌肉功能。本单元描述了一个协议的尸检，处理，冷冻切片，和组织病理学评价的小鼠骨骼肌，这是适用于两种模型的肌营养不良症和其他神经肌肉条件。以themdx小鼠（杜氏肌营养不良模型）为例，讨论了营养不良性肌肉的关键组织病理学特征。解剖、处理和切片过程中的最佳处理对于避免可能混淆或妨碍未来分析的伪影至关重要。使用该方案仔细处理的肌肉适合于使用免疫组织化学、免疫荧光、特殊组织化学染色和免疫印迹进行进一步评价。John Wiley & Sons，Inc.
The muscular dystrophies are a diverse group of degenerative diseases for which many mouse models are available. These models are frequently used to assess potential therapeutic interventions and histological evaluation of multiple muscles is an important part of this assessment. Histological evaluation is especially useful when combined with tests of muscle function. This unit describes a protocol for necropsy, processing, cryosectioning, and histopathological evaluation of murine skeletal muscles, which is applicable to both models of muscular dystrophy and other neuromuscular conditions. Key histopathological features of dystrophic muscle are discussed using themdxmouse (a model of Duchenne muscular dystrophy) as an example. Optimal handling during dissection, processing and sectioning is vital to avoid artifacts that can confound or prevent future analyses. Muscles carefully processed using this protocol are suitable for further evaluation using immunohistochemistry, immunofluorescence, special histochemical stains, and immuoblotting. © 2016 by John Wiley & Sons, Inc.