Acupuncture improves cognitive deficits and regulates the brain cell proliferation of SAMP8 mice
Acupuncture improves cognitive deficits and regulates the brain cell proliferation of SAMP8 mice
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针灸改善 SAMP8 小鼠的认知缺陷并调节脑细胞增殖
DOI：
10.1016/j.neulet.2007.12.009
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发表时间：
2008-02-20
影响因子：
2.5
通讯作者：
Han, Jingxian
中科院分区：
文献类型：
--
作者：
Cheng, Haiyan;Yu, Jianchun;Han, Jingxian
衰老加速小鼠易感8 （SAMP8）是一种以早期认知障碍和与年龄相关的学习和记忆退化为特征的自源性衰老菌株。为了研究针刺对4月龄雄性SAMP8和年龄匹配的同源正常衰老SAMR1小鼠行为改变和脑细胞事件的影响，将其分为SAMP8针刺组（Pa）、SAMP8非穴位对照组（Pn）、SAMP8对照组（Pc）和SAMR1正常对照组（Rc） 4组。Morris水迷宫实验显示SAMP8认知缺陷，益气调血复本培元针刺可显著改善SAMP8认知缺陷。同时，通过5'-溴-2'-脱氧尿苷（BrdU）特异性免疫检测，针刺治疗可显著增强SAMP8齿状回（DG）细胞增殖下降，提示穴位相关特异性。尽管三脑室（V3）和侧脑室（LV）的心室/室下区（VZ/SVZ）在两组间没有明显差异，但我们得到了有趣的结果：新增殖的细胞沿海马肺泡背（Alv）呈流状分布，从LV延伸到胼胝体（CC），治疗针刺对该区域有明显的影响。我们的研究表明，针刺可诱导SAMP8脑不同区域的不同细胞增殖，这就需要进一步探索认知缺陷的机制和针刺在该领域的干预。2007爱思唯尔爱尔兰有限公司版权所有。
Senescence-accelerated mouse prone 8 (SAMP8) is an autogenic senile strain characterized by early cognitive impairment and age-related deterioration of learning and memory. To investigate the effect of acupuncture on behavioral changes and brain cell events, male 4-month-old SAMP8 and age-matched homologous normal aging SAMR1 mice were divided into four groups: SAMP8 acupuncture group (Pa), SAMP8 non-acupoint control group (Pn), SAMP8 control group (Pc) and SAMR1 normal control group (Rc). By Morris water maze test, the cognitive deficit of SAMP8 was revealed and significantly improved by "Yiqitiaoxue and Fubenpeiyuan" acupuncture. Meanwhile, by 5'-bromo-2'-deoxyuridine (BrdU) specific immunodetection, the decreased cell proliferation in dentate gyrus (DG) of SAMP8 was greatly enhanced by the therapeutic acupuncture, suggesting acupoint-related specificity. Even though no significant differences were found in ventricular/subventricular zones (VZ/SVZ) of the third ventricle (V3) and lateral ventricle (LV) between groups, we obtained interesting results: a stream-like distribution of newly proliferated cells presented along the dorsum of alveus hippocampi (Alv), extending from LV to corpus callosum (CC), and the therapeutic acupuncture showed a marked effect on this region. Our research suggests that acupuncture can induce different cell proliferation in different brain regions of SAMP8, which brings forth the need to explore further for the mechanism of cognitive deficits and acupuncture intervention in this field. (c) 2007 Elsevier Ireland Ltd. All rights reserved.