Simultaneous determination of neutral and amino sugars in biological materials.
Simultaneous determination of neutral and amino sugars in biological materials.
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同时测定生物材料中的中性糖和氨基糖。
DOI：
10.1016/s0021-9673(01)89426-7
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Marlett,JA
中科院分区：
文献类型：
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作者：
Kraus,RJ;Shinnick,FL;Marlett,JA
本文介绍了一种同时分析九种中性糖和三种氨基糖的方法。用两步Saeman程序酸水解标准糖和生物样品的混合物，用BaCO 3中和，用硼氢化钠还原，酸化，蒸发并制备糖醇乙酸酯。在玻璃毛细管SP-2340柱上，在52 min内实现基线分辨率。测定重现性、响应因子和水解损失。定量在10-20 μg/ml至2000 μg/ml范围内呈线性。定义了胞壁酸可重复定量的条件。
A method is described for the simultaneous analysis of nine neutral and three amino sugars. Mixtures of standard sugars and biological samples were acid hydrolyzed with a two step Saeman procedure, neutralized with BaCO3, reduced with sodium borohydride, acidified, evaporated and alditol acetates prepared. Baseline resolution was achieved on a glass-capillary SP-2340 column inca.52 min. Reproducibility, response factors and hydrolysis losses were determined. Quantitation was linear over the range of 10–20 μg/ml to 2000 μg/ml. Conditions were defined for the reproducible quantitation of muramic acid.