Calcium regulation of vasoactive intestinal polypeptide mRNA abundance in SH-SY5Y human neuroblastoma cells.
Calcium regulation of vasoactive intestinal polypeptide mRNA abundance in SH-SY5Y human neuroblastoma cells.
复制标题
SH-SY5Y 人神经母细胞瘤细胞中血管活性肠多肽 mRNA 丰度的钙调节。
DOI：
10.1111/j.1471-4159.1993.tb02179.x
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发表时间：
1993
影响因子：
4.7
通讯作者：
Fink,JS
中科院分区：
文献类型：
--
作者：
Adler,EM;Fink,JS
Second messenger regulation of gene expression provides a mechanism by which neurons can transduce environmental stimuli into long‐term changes in the expression of molecules involved in neuronal signaling. We have investigated calcium‐dependent induction of vasoactive intestinal polypeptide (VIP) mRNA and compared it with induction of VIP mRNA by cyclic AMP. Depolarization with 60mMKCI or exposure to the calcium ionophore A23187 increases VIP mRNA levels in SH‐SY5Y cells. The increase in VIP mRNA content in response to Ca2+mobilization is slow, independent of adenylate cyclase activation, and requires de novo protein synthesis. The increase in VIP mRNA content in response to elevation of cyclic AMP levels by forskolin/isobutylmethylxanthine is independent of Ca2+influx and does not require new protein synthesis. The mRNA for the transcription factors ATF‐3, c‐fos,c‐jun, junB, andzif/268is induced by A23187. Of these, ATF‐3 showed the greatest relative induction by A23187 compared with induction by forskolin/isobutyl‐methylxanthine.
