Amino acid supplementation improves heterologous protein production by Saccharomyces cerevisiae in defined medium
Amino acid supplementation improves heterologous protein production by Saccharomyces cerevisiae in defined medium
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DOI：
10.1007/s00253-004-1803-3
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发表时间：
2005-06-01
影响因子：
5
通讯作者：
Hahn-Hägerdal, B
中科院分区：
文献类型：
--
作者：
Görgens, JF;van Zyl, WH;Hahn-Hägerdal, B
研究了在化学成分确定的培养基中添加氨基酸或琥珀酸以提高原养型酿酒酵母产异源木聚糖酶的能力。在分批培养和葡萄糖限制恒化培养中在乙醇上生长期间的相应木聚糖酶产生被定量，因为调节木聚糖酶表达的天然ADH 2启动子在这些条件下被去阻遏。优选氨基酸Ala、Arg、Asn、Glu、Gln和Gly的平衡混合物的添加改善了生物质和木聚糖酶的产生，而几种其它单独的氨基酸抑制了生物质和/或木聚糖酶的产生。通过向培养基中补充琥珀酸，重组酵母的杂合蛋白生产也得到改善。因此，在乙醇或葡萄糖上生长期间异源木聚糖酶的产生可以通过在碳或氮代谢中补充代谢前体来改善。
Supplementation of a chemically defined medium with amino acids or succinate to improve heterologous xylanase production by a prototrophic Saccharomyces cerevisiae transformant was investigated. The corresponding xylanase production during growth on ethanol in batch culture and in glucose-limited chemostat culture were quantified, as the native ADH2 promoter regulating xylanase expression was derepressed under these conditions. The addition of a balanced mixture of the preferred amino acids, Ala, Arg, Asn, Glu, Gln and Gly, improved both biomass and xylanase production, whereas several other individual amino acids inhibited biomass and/or xylanase production. Heterologous protein production by the recombinant yeast was also improved by supplementing the medium with succinate. The production of heterologous xylanase during growth on ethanol or glucose could thus be improved by supplementing metabolic precursors in the carbon- or nitrogen-metabolism.