Acute effects of PYY3-36 on food intake and hypothalamic neuropeptide expression in the mouse
Acute effects of PYY3-36 on food intake and hypothalamic neuropeptide expression in the mouse
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DOI：
10.1016/j.bbrc.2003.10.089
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发表时间：
2003-11-28
影响因子：
3.1
通讯作者：
O'Rahilly, S
中科院分区：
文献类型：
--
作者：
Challis, BG;Pinnock, SB;O'Rahilly, S
最近有研究表明，肠道来源的PYY3-36可能参与了餐后饱腹感信号的中枢调解。我们研究了外周给药PYY3-36对小鼠食物摄入和下丘脑神经肽基因表达的急性影响。对禁食24小时的小鼠单次腹腔注射PYY3-36，在注射后6和24小时的食物摄入量显著减少，但在48小时没有。然而，在自由喂养的小鼠中，PYY3-36的摄入没有改变。PYY3-36注射后6 h POMC mRNA表达显著升高，24 h POMC mRNA表达持续升高。相比之下，注射后6 h弓形核NPY mRNA表达受到抑制，24 h无抑制。在下丘脑外侧，MCH mRNA在两个时间点的表达均无差异。总之，外周给药PYY3-36对食物摄入的抑制作用在最近禁食的小鼠中更为突出，持续时间长达24小时。这与NPY mRNA的短期抑制，POMC mRNA的持续增加而MCH mRNA的表达没有变化有关。(C) 2003 Elsevier Inc.版权所有。
It has recently been suggested that gut-derived PYY3-36 may be involved in the central mediation of post-prandial satiety signals. We have examined the acute effects of peripherally administered PYY3-36 on food intake and hypothalamic gene expression of neuropeptides in mice. A single intraperitoneal injection Of PYY3-36 to mice that had been fasted for 24 h resulted in a highly significant reduction in food intake at 6 and 24 h post-injection but not at 48 h. However, in freely fed mice, food intake was unaltered by PYY3-36 administration. In the arcuate nucleus POMC mRNA expression was significantly elevated at 6 h and remained elevated at 24 h following PYY3-36 injection. By contrast NPY mRNA expression in the arcuate nucleus was suppressed at 6 h but not at 24 h post-injection. In the lateral hypothalamus there were no differences in MCH mRNA expression at either time point. In conclusion, peripherally administered PYY3-36 has a suppressive effect on food intake that is more prominent in recently fasted mice and lasts up to 24 h. This is associated with a short-lived suppression of NPY mRNA, a longer lasting increase in POMC mRNA but no change in MCH mRNA expression. (C) 2003 Elsevier Inc. All rights reserved.