MiR-3910 Promotes the Growth and Migration of Cancer Cells in the Progression of Hepatocellular Carcinoma
MiR-3910 Promotes the Growth and Migration of Cancer Cells in the Progression of Hepatocellular Carcinoma
复制标题
DOI：
10.1007/s10620-017-4670-3
复制
发表时间：
2017-10-01
影响因子：
3.1
通讯作者：
Han, Shuangyin
中科院分区：
文献类型：
--
作者：
Cheng, Lina;Wang, Hongwei;Han, Shuangyin
以往的研究报道，小鼠肝脏中哺乳动物不育样激酶（MST 1）的特异性缺失可导致肝细胞癌（HCC）的发生。本研究采用q-PCR技术检测miR-3910在肝癌组织和细胞系中的表达。采用MTT法、Boyden小室法和软琼脂法检测miR-3910在HCC中的作用。在小鼠肝癌转移模型中，我们研究了miR-3910对肝癌细胞转移的影响，发现miR-3910调控MST 1的表达。miR-3910在HCC样品和细胞系中上调，并且miR-3910的表达由致癌的RasV 12诱导。在功能研究中，发现miR-3910能促进肝癌细胞的生长和迁移，敲低miR-3910能抑制肝癌细胞的转移。研究结果表明，miR-3910在肝癌发生发展过程中具有致癌作用，可能成为肿瘤治疗的一个新靶点。
Previous studies have reported that specific depletion of mammalian sterile-like kinase (MST1) in the mouse liver driven Hepatocellular carcinoma (HCC). However, how the expression of MST1 was regulated in the progression of HCC remains largely unknown.The expression of miR-3910 in the HCC tissues and cell lines were examined using q-PCR. The functions of miR-3910 in HCC were examined using MTT assay, Boyden chamber assay and soft agar assay. The effects of miR-3910 on the metastasis of HCC cells were evaluated using the mouse model.Here, we have shown that miR-3910 regulated the expression of MST1. MiR-3910 was up-regulated in HCC samples and cell lines, and the expression of miR-3910 was induced by the oncogenic RasV12. In the functional study, miR-3910 was found to promote the growth and migration of HCC cells, and knocking down miR-3910 inhibited the metastasis of HCC cells. Mechanically, it was found that miR-3910 activated YAP signaling by targeting MST1.Taken together, this study demonstrated that miR-3910 exerted oncogenic effects on the progression of HCC and suggested that miR-3910 might be a therapeutic target for cancer therapy.