RNA signatures allow rapid identification of pathogens and antibiotic susceptibilities
RNA signatures allow rapid identification of pathogens and antibiotic susceptibilities
复制标题
DOI：
10.1073/pnas.1119540109
复制
发表时间：
2012-04-17
影响因子：
11.1
通讯作者：
Hung, Deborah T.
中科院分区：
文献类型：
--
作者：
Barczak, Amy K.;Gomez, James E.;Hung, Deborah T.
随着耐药感染率的上升，需要能够快速检测病原体的存在并揭示其对抗生素敏感性的诊断方法。在这里，我们提出了一种基于直接检测临床样本RNA的方法来诊断传染病的存在和药物敏感性。我们证明，物种特异性RNA标记可用于识别广泛的感染因子，包括细菌，病毒，酵母和寄生虫。此外，我们表明，一小部分细菌转录本在短暂的抗生素脉冲后的行为可以迅速区分药物敏感和耐药生物，这些测量可以直接从临床材料中进行。因此，转录特征可以形成适用于广泛感染因子的统一诊断平台的基础。
With rising rates of drug-resistant infections, there is a need for diagnostic methods that rapidly can detect the presence of pathogens and reveal their susceptibility to antibiotics. Here we propose an approach to diagnosing the presence and drug-susceptibility of infectious diseases based on direct detection of RNA from clinical samples. We demonstrate that species-specific RNA signatures can be used to identify a broad spectrum of infectious agents, including bacteria, viruses, yeast, and parasites. Moreover, we show that the behavior of a small set of bacterial transcripts after a brief antibiotic pulse can rapidly differentiate drug-susceptible and -resistant organisms and that these measurements can be made directly from clinical materials. Thus, transcriptional signatures could form the basis of a uniform diagnostic platform applicable across a broad range of infectious agents.