Measurement of pH Distribution in Microchannel Flow Using 2-Color Laser-induced Fluorescence
Measurement of pH Distribution in Microchannel Flow Using 2-Color Laser-induced Fluorescence
复制标题
使用 2 色激光诱导荧光测量微通道流中的 pH 分布
DOI：
10.1299/kikaib.71.2964
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发表时间：
2005
期刊：
影响因子：
--
通讯作者：
Kazuyuki Nakamura
中科院分区：
文献类型：
--
作者：
S. Saeki;M. Kaneta;Takashi Saito;T. Nishida;Kazuyuki Nakamura
提出了一种新型的微流体pH测量技术；“双色激光诱导荧光”。在该技术中，通过将一个荧光图像与同时拍摄的另一个荧光图像分开，可以消除激发光分布的不良影响。这可以简化测量过程，因为在许多图像位置省略了荧光强度归一化，并且比单色LIF更准确地测量标量分布。本研究将该方法应用于t型微通道中的化学反应中和。使用荧光素和罗丹明B，用7种不同的pH溶液校准荧光强度的分割值。与单色LIF图像相比，空间强度误差可降低至约80 - 90%。在一定流速条件下，定量地观察了t结处醋酸与氢氧化氨中和反应的pH分布。
This paper presents a novel pH measurement technique for microfluidic devices; "2-Color Laser-induced Fluorescence". In this technique, an undesirable influence of excitation light distribution can be eliminated by dividing one fluorescence image with the other one taken at the same time. This can simplify measurement procedure in terms of omitting fluorescence intensity normalization at a lot of image locations, and measure a scalar distribution more accurately than 1-color LIF. In this study, the proposed method was applied to neutralization chemical reaction in T-type microchannel. Using Fluorescein and Rhodamine B, the divided value of fluorescence intensities was calibrated with 7 different pH solutions. Spatial intensity error can be reduced to about 80 to 90% as compared with 1-color LIF image. As a result, pH distribution of neutralization reaction between acetic acid and ammonia hydroxide at T-junction was visualized quantitatively at some flow rate conditions.