The mitogen-activated protein kinase cascade MKK1-MPK4 mediates salt signaling in rice.
The mitogen-activated protein kinase cascade MKK1-MPK4 mediates salt signaling in rice.
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DOI：
10.1016/j.plantsci.2014.08.007
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发表时间：
2014-10
期刊：
影响因子：
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通讯作者：
Fuzheng Wang;Wen Jing;Wenhua Zhang
中科院分区：
文献类型：
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作者：
Fuzheng Wang;Wen Jing;Wenhua Zhang
有丝分裂原活化蛋白激酶（MAPK）信号通路参与植物生物和非生物胁迫的信号转导。在本研究中，我们发现，水稻（Oryza sativa）MAPKK（OsMKK 1）的转录显着增加盐胁迫。通过检测OsMKK 1基因敲除突变体的存活率和地上部Na+含量，发现OsMKK 1基因敲除突变体比野生型对盐胁迫更敏感。盐胁迫提高了野生型幼苗和原生质体中OsMKK 1的活性。酵母双杂交和体外及体内激酶试验表明OsMKK 1靶向OsMPK 4。OsMPK 4活性在盐胁迫下升高，但盐胁迫对OsMPK 4活性有抑制作用。相反，OsMKK 1的过表达增加了原生质体中OsMPK 4的活性。通过比较野生型和突变型OsMKK1的转录因子水平，OsMKK1是盐诱导OsDREB2和OsMYBS3表达增加所必需的。综上所述，数据表明OsMKK 1和OsMPK 4构成了调节水稻耐盐性的信号通路。
Mitogen-activated protein kinase (MAPK) pathways have been implicated in signal transduction of both biotic and abiotic stresses in plants. In this study, we found that the transcript of a rice (Oryza sativa)MAPKK(OsMKK1) was markedly increased by salt stress. By examining the survival rate and Na+content in shoot, we found thatOsMKK1-knockout (osmkk1) mutant was more sensitive to salt stress than the wild type. OsMKK1 activity in the wild-type seedlings and protoplasts was increased by salt stress. Yeast two-hybrid andin vitroandin vivokinase assays revealed that OsMKK1 targeted OsMPK4. OsMPK4 activity was increased by salt, which was impaired inosmkk1plants. In contrast, overexpression ofOsMKK1increased OsMPK4 activity in protoplasts. By comparing the transcription factors levels between WT andosmkk1mutant,OsMKK1was necessary for salt-induced increase inOsDREB2BandOsMYBS3. Taken together, the data suggest that OsMKK1 and OsMPK4 constitute a signaling pathway that regulates salt stress tolerance in rice.