Possible use of similar framework region amino acid sequences between human and mouse immunoglobulins for humanizing mouse antibodies.
Possible use of similar framework region amino acid sequences between human and mouse immunoglobulins for humanizing mouse antibodies.
复制标题
可能使用人和小鼠免疫球蛋白之间相似的框架区氨基酸序列来人源化小鼠抗体。
DOI：
10.1016/0161-5890(92)90047-2
复制
发表时间：
1992
影响因子：
3.6
通讯作者：
Kabat,EA
中科院分区：
文献类型：
--
作者：
Wu,TT;Kabat,EA
我们之前已经注意到，一个特定的氨基酸序列可以形成人类、小鼠和兔子免疫球蛋白轻链的第二框架区域，这表明该序列已经保存了8000万年。通过分化进化，每个物种获得了一套不同的框架区序列；然而，这些组合仍然有一些相似或相同的氨基酸序列。在目前的研究中，我们已经确定了人类和小鼠免疫球蛋白轻链和重链之间的所有四个框架区域的序列。它们可能有助于人源化或重塑小鼠或大鼠抗体，用于人类患者的治疗应用。
We have previously noted that a specific amino acid sequence could form the second framework region of human, mouse and rabbit immunoglobulin light chains, suggesting that this sequence has been preserved for 80 million years. Through divergent evolution, each species has acquired a different set of framework region sequences; however, these sets still share a few similar or identical amino acid sequences. In the present study, we have identified such sequences for all four framework regions between human and mouse immunoglobulin light and heavy chains. They may be useful in humanizing or reshaping mouse or rat antibodies for therapeutic applications in human patients.