Microfabricated multi-frequency particle impedance characterization system
Microfabricated multi-frequency particle impedance characterization system
复制标题
微加工多频粒子阻抗表征系统
DOI：
10.1007/978-94-017-2264-3_60
复制
发表时间：
2000
影响因子：
2.4
通讯作者：
P. Gascoyne
中科院分区：
文献类型：
--
作者：
C. Fuller;J. Hamilton;H. Ackler;P. Krulevitch;B. Boser;A. Eldredge;F. Becker;Jun Yang;P. Gascoyne
我们开发了一种微型制造的流动阻抗表征系统，能够对细胞和其他颗粒进行交流，多频率测量。该传感器以每秒100个粒子的速率测量通过粒子的电阻阻抗和反应阻抗。其工作带宽接近10 MHz，信噪比约为40 dB。粒子阻抗在三个或更多频率下同时测量，从而可以推导多个粒子参数。这是对通过库尔特原理建立的直流颗粒分级技术的改进。测定了人外周血粒细胞半径、膜电容和细胞质电导率（r = 4.1 μm, Cmem = 0.9 μF/cm2, σint = 0.66 S/m），结果与文献数据一致。
We have developed a microfabricated flow-through impedance characterization system capable of performing AC, multi-frequency measurements on cells and other particles. The sensor measures both the resistive and reactive impedance of passing particles, at rates of up to 100 particles per second. Its operational bandwidth approaches 10 MHz with a signal-to-noise ratio of approximately 40 dB. Particle impedance is measured at three or more frequencies simultaneously, enabling the derivation of multiple particle parameters. This constitutes an improvement to the well-established technique of DC particle sizing via the Coulter Principle. Human peripheral blood granulocyte radius, membrane capacitance, and cytoplasmic conductivity were measured (r = 4.1 μm, Cmem = 0.9 μF/cm2, σint = 0.66 S/m) and were found to be consistent with published values.