Microbial production of neryl-α-D-glucopyranoside from nerol by Agrobacterium sp. M-12 reflects glucosyl transfer activity
Microbial production of neryl-α-D-glucopyranoside from nerol by Agrobacterium sp. M-12 reflects glucosyl transfer activity
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橙花醇-α-D-吡喃葡萄糖苷的微生物生产反映了农杆菌 M-12 葡萄糖基转移活性。
DOI：
10.1080/09168451.2018.1514250
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Ueda Makoto
中科院分区：
文献类型：
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作者：
Takahashi Kazuki;Terauchi Issei;Ono Marie;Satoh Hiroshi;Ueda Makoto
萜烯醇广泛用于香水中，并具有抗菌活性。此外，在其糖基化形式下，它可以作为非离子表面活性剂应用于食品，制药，化工，化妆品和洗涤剂行业。目前，萜类苷的化学生产存在成本高、收率低的问题。本文研究了农杆菌对挥发油的一种组分——络醇（顺式-3,7-二甲基-2,6-二烯-1-醇）的微生物糖基化作用。M-12从土壤中分离出来。用农杆菌洗过的细胞进行的微生物反应。在M-12、1 g/L橙花醇和100 g/L麦芽糖的最优条件下，72 h后，橙花基-α-d-葡萄糖苷的摩尔产率为1.8 g/L，产率为87.6%。此外，我们还报道了农杆菌成功地将香叶醇、(-)-β-香茅醇和(-)-芳樟醇等其他单萜醇进行糖基化。M-12。因此，微生物糖基化对于高效、低成本生产糖基化萜烯醇具有潜在的广泛适用性。
Terpene alcohol is widely used in perfumes and is known to possess antibacterial activity. Moreover, in its glycosylated form, it can be applied as a nonionic surfactant in food, and in the pharmaceutical, chemical, cosmetic, and detergent industries. Presently, chemical production of terpene glucosides is hampered by high costs and low yields. Here, we investigated the microbial glucosylation of nerol (cis-3,7-dimethylocta-2,6-dien-1-ol), a component of volatile oils, byAgrobacteriumsp. M-12 isolated from soil. A microbial reaction using washed cells ofAgrobacteriumsp. M-12, 1 g/L of nerol, and 100 g/L of maltose under optimal conditions yielded 1.8 g/L of neryl-α-d-glucopyranoside after 72 h. The molar yield of neryl-α-D-glucopyranoside was 87.6%. Additionally, we report the successful transglucosylation of other monoterpene alcohols, such as geraniol, (-)-β-citronellol, and (-)-linalool, byAgrobacteriumsp. M-12. Thus, microbial glucosylation has potential widespread applicability for efficient, low-cost production of glycosylated terpene alcohols.